TABLE 1.
Background proteinsa
| Protein ID | Protein description | Normalized level (%) |
|---|---|---|
| AATM_HUMAN | Aspartate aminotransferase | 0.9 |
| ACACA_HUMAN | Acetyl-CoA carboxylase | 28.9 |
| ACTIN* | Actins | 1.7 |
| ADT2_HUMAN | ADP/ATP translocase | 0.9 |
| ALDOA_HUMAN | Fructose-bisphosphate aldolase | 1.2 |
| ANXA2_HUMAN | Annexin A2 | 1.0 |
| APEX1_HUMAN | DNA lyase | 0.8 |
| C1TC_HUMAN | C-1-tetrahydrofolate synthase | 1.1 |
| CAH2_HUMAN | Carbonic anhydrase | 1.0 |
| HS PROTEINS* | Heat shock proteins | 2.0 |
| TRYPSIN | Trypsin precursor | 4.6 |
| KERATIN* | Keratins | 26.8 |
| DCD_HUMAN | Dermcidin | 3.9 |
| DESP_HUMAN | Desmoplakin | 5.4 |
| DSC1_HUMAN | Desmocollin-1 | 1.4 |
| DSG1_HUMAN | Desmoglein-1 | 2.3 |
| EF* | Elongation factors | 4.9 |
| ENOA_HUMAN | Alpha-enolase | 4.7 |
| FUBP2_HUMAN | Far upstream element BP | 1.7 |
| G3P_HUMAN | G-3-phosphate dehydrogenase | 2.5 |
| GBLP_HUMAN | Guanine nucleotide BP | 2.4 |
| GRP75_HUMAN | Stress-70 protein | 1.8 |
| GRP78_HUMAN | 78-kDa glucose-regulated protein | 2.8 |
| HNRPK_HUMAN | Het nuclear ribonucleoprotein K | 2.2 |
| HUWE1_HUMAN | E3 ubiquitin-protein ligase | 1.1 |
| IGHA1_HUMAN | Ig alpha-1 chain C region | 1.5 |
| KPYM_HUMAN | Pyruvate kinase | 2.9 |
| LDH* | l-Lactate dehydrogenases | 1.0 |
| MCCA_HUMAN | Methylcrotonoyl-CoA carboxylase | 9.6 |
| MDHM_HUMAN | Malate dehydrogenase | 1.3 |
| MOES_HUMAN | Moesin | 1.0 |
| NUCL_HUMAN | Nucleolin | 1.9 |
| PARP1_HUMAN | Poly[ADP-ribose] polymerase | 2.0 |
| PCCA_HUMAN | Propionyl-CoA carboxylase | 29.6 |
| PGK1_HUMAN | Phosphoglycerate kinase | 2.2 |
| PLAK_HUMAN | Junction plakoglobin | 3.4 |
| PLST_HUMAN | Plastin-3 | 0.9 |
| PPIA_HUMAN | Peptidyl-prolyl isomerase A | 1.8 |
| PERIREDOXIN* | Peroxiredoxins | 1.1 |
| PYC_HUMAN | Pyruvate carboxylase | 31.9 |
| PYR1_HUMAN | CAD protein | 0.8 |
| RAN_HUMAN | GTP-binding protein Ran | 1.3 |
| RCC2_HUMAN | Protein RCC2 | 1.1 |
| 60S PROTEIN* | 60S ribosomal proteins | 0.9 |
| SAHH_HUMAN | Adenosylhomocysteinase | 0.9 |
| TUBULIN | Tubulins | 1.8 |
| TGM3_HUMAN | Protein glutamyltransferase | 1.2 |
| TKT_HUMAN | Transketolase | 1.2 |
| TLN1_HUMAN | Talin-1 | 0.9 |
| TPIS_HUMAN | Triosephosphate isomerase | 1.4 |
Proteins from MS analyses were designated background proteins as described in Materials and Methods and include endogenously biotinylated proteins and standard artifacts. The left column gives the UniProt ID name; the center column gives the protein description; the right column gives the MS signals, normalized as percentages relative to the summed signals of acetyl-CoA carboxylase plus methylcrotonyl-CoA carboxylase plus proprionyl-CoA carboxylase plus pyruvate carboxylase and averaged over all MS runs. The asterisks indicate that the values given represent normalized averaged signals for the following: actins (ACTA and ACTB), heat shock proteins (CH60, HSL71L, HS90A, HS90B, HSP71, HSP7C, and TRAP1), keratins (K1C2, K1C9, K1C10, K1C13, K1C14, K1C17, K22E, K2C1, K2C1B, K2C5, K2C8, K2C78, KRT5, KRT8, KRT10, and Q13092), elongation factors (EF1A1, EF2, and EFTU), lactate dehydrogenases (LDHA and LDHB), peridoxins (PRDX1 and PRDX2), 60S ribosomal proteins (RL5 and RL6), and tubulins (TBA1A, TBA1B, TBA1C, TBB1, TBB4B, and TBB5).