FIG 7.

Inhibition of RSV replication by P[1-29]. (A) BSRT7/5 cells were transfected with pP, pN, pM2-1, pL plasmids, and pM/Luc, together with pCMV-βGal for transfection standardization, and various ratios of pP[1-29]/pP or pmCherry-P[1-29]/pP. (B) Comparison of Luc activity using pM/Luc and pMΔ/Luc vectors coding for replication-competent and replication-defective minigenomes, respectively. (C) Replication-defective minigenome assay with a pmCherry-P[1-29]/pP ratio of 1.5. Viral RNA synthesis was quantified by measuring the Luc activity after cell lysis 24 h after transfection. Each Luc activity value was normalized based on β-galactosidase expression and is the average of results from three independent experiments performed in duplicate. Error bars represent standard deviations calculated based on three independent experiments made in duplicate.