TABLE 1.
Oligonucleotides used in the study
| Target sequence | Primer | Sequence (5′–3′) | Length (bp) |
|---|---|---|---|
| ORF73 | ORF73-3598F | GGACTAGACCCTCTTTATGACCC | 244 |
| ORF73-3841R | CGAGCAAAATAGGAACCCATTGC | ||
| ORF09 (DPOL) | ORF09-2170F | GTAACCCTGCAAGGCAGAACC | 469 |
| ORF09-2638R | GTGAATAGGAAAGGGTCTGG | ||
| ORF22 (gH) | ORF22-239F | GTTTACTGATCAATCGATAAGAG | 952 |
| ORF22-1190R | CATCCACAAAGTACAGTGTCTGC | ||
| A3 | A3142S | GACATGGGCACTTTGTGTGTTAG | |
| A3712AS | GGCCGGTGCATGGCGGTGTC | 570 | |
| A3-Fc | XbaIA3142S | TCTAGAATGGGCACTTTGTGTGTTAG | 1,824 |
| A3deltastop | GACATCAGTGTCACATTCACCTC | ||
| A3-V5 | A3142S | GACATGGGCACTTTGTGTGTTAG | 1,818 |
| A3deltastop#2 | CTCGAGCATCAGTGTCACATTCACCTC | ||
| NotIA3V5S | CAGGAGCTCGCGGCCATGGGCACTTTGTGTGTTAG | 1,966 | |
| NotIA3V5AS | CGATGGGATCCGCGGCCTCATCAATGGTGATGGTGATGATG | ||
| A3-galK | SEMAFgalkF | GACACACCCTGACACCCAACACTGCTCAATGTCTACTTAACATGTAGTATCATCAATCCTGTTGACAATTAATCATCGGCA | 1,345 |
| SEMAFgalkR | GGTGAGTTTTTAGATACATCATCCACTTGTAAGACAATGTTGATAACAAGGACTGTGTCAGCACTGTCCTGCTCCTT | ||
| A3-rev | SEMAF | ACACACCCTGACACCCAACACTGCTCAATGTCTACTTAACATGTAGTATCATCAAT | 2,074 |
| SEMAR | GGTGAGTTTTTAGATACATCATCCACTTGTAAGACAATGTTGATAACAAGGACTGTG | ||
| A3-ns-galK | A3-NS-galkFwd | GTTTGGGGCTGATAACAGAGTTTGTAATACATCCTGCAGCTATGGGCACTCCTGTTGACAATTAATCATCGGCA | 1,331 |
| A3-NS-galkRev | GATTTAGCACTGTGATGGC TGATAAAATCATCAGTAATCTAATACTAACTCAGCACTGTCCTGCTCCTT | ||
| A3-ns | A3-NS-oligofwd | CTGATAACAGAGTTTGTAATACATCCTGCAGCTATGGGCACTTAGTGATGAATTCGTTAGTATTAGATTACTGATGATTTTATCAGCCATCACAGCTG | 98 |
| A3-NS-oligorev | CAGCTGTGATGGCTGATAAAATCATCAGTAATCTAATACTAACGAATTCATCACTAAGTGCCCATAGCTGCAGGATGTATTACAAACTCTGTTATCAG | ||
| A3-ns-rev | preH1A3S | GCAAGAGTGTACCATGTCTCC | 597 |
| A3407AS | GGTGGGTATTATGTGTGCTAG | ||
| A3-end-galK | SemaendgalKF | GAAGATTCGGTGACAGTGAAACTGTTAGAGGTGAATGTGACACTGATGCCTGTTGACAATTAATCATCGGCA | 1,331 |
| SemaendgalKR | GTTTTTAGATACATCATCCACTTGTAAGACAATGTTGATAACAAGGACTGTGTCAGCACTGTCCTGCTCCTT | ||
| A3-Fc | SemaFcF | GAAGATTCGGTGACAGTGAAACTGTTAGAGGTGAATGTGACACTGATGTCAATCACTAGTGAATTCGCGGCCGCG | 858 |
| SemaFcR | GTTTTTAGATACATCATCCACTTGTAAGACAATGTTGATAACAAGGACTGTGTCATTTACCCGGAGACAGGGAGAGGC |