FIG 4.

Anti-apoE antibody neutralization. Wild-type JFH1 and JFH1-ΔHVR1 viruses were preincubated with either control MAb RO4 (C), polyclonal antibody (E), or different anti-apoE antibodies, MAb E3 (A), MAb 23 (B), or polyclonal antibody 947 (D), at 37°C for 1 h and then added to the cells for 2 h. The inoculum was removed, and the cells were washed and incubated for another 30 h. Infected cells were detected by indirect immunofluorescence. Results are expressed as the percentage of HCVcc infectivity in the absence of antibodies. Mean values from three independent experiments are given. Error bars represent the standard deviations of the means.