FIG 6.

apoE mediates HCV binding to HS. (A) Purified H77/JFH1 chimera was preincubated for 1 h at 37°C with 25 μg/ml heparin, antibodies (5 μg/ml MAb AR3A, 10 μg/ml MAb AR5A, 10 μg/ml MAb RO4, 10 μg/ml MAb 6/16, 20 μl MAb 9/27, 100 μg/ml purified IgG from a control patient [patient 5] or four different gt1a-infected patients [patients 1 to 4], 1/200-diluted anti-apoE polyclonal antibodies, or irrelevant [control] polyclonal antibodies [Ctrl pAb]), 20 μg/ml apoE-derived peptides, or 3× Flag-tagged control peptides before heparin-coated beads were added. The amount of virus that bound to the beads was measured by quantitative RT-PCR. The quantity of virus bound to the beads in the absence of any competitor was arbitrarily set equal to 100%. Mean values from three independent experiments are given. Data were analyzed by using a Dunnett's multiple-comparison test (***, P < 0.001). (B) Purified JFH1 or JFH1-ΔHVR1 viruses were incubated for 1 h at 37°C in the absence or presence of 25 μg/ml heparin and then precipitated with anti-apoE antibody-coated beads. Virus bound to the beads was measured by quantitative RT-PCR. Mean values from three independent experiments are given. Error bars represent the standard deviations of the means.