FIG 11.

GST-p18 interacts with endogenous Ty1 Gag. Protein extracts (Input) from BY4742 induced for expression of GST (DG3808) or GST-p18 (DG3809) were incubated with glutathione-coated resin. Bound proteins were analyzed by immunoblotting to detect Gag, GST-p18, and p18/Ty1 Gag complexes (Pull-down) after extensive washing with lysis buffer. A Ty1-less strain expressing GST-p18 (DG3810) and the presence of Hts1 served as negative controls. Gag was detected with TY tag monoclonal antibody, which recognizes p49/p45 but not p22/p18 due to the location of the epitope. GST proteins and Hts1 were detected with GST and Hts1 antibodies, respectively.