FIG 7.
Cleavage of p22 to p18 does not disturb trans-dominant inhibition of Ty1 mobility. A mutant Gag-PR cleavage site, AAGSAA (Gag*PR) (40), was inserted into p22, replacing the normal Gag-PR cleavage site, RAHNVS. A Ty1-less strain containing pGTy1his3-AI and an empty vector (DG3739; lane 1), GAL1-p22 (DG3774; lane 2), GAL1-p18 (DG3791; lane 3), or GAL1-p22Gag*PR (JM399; lane 4) was analyzed for Ty1his3-AI mobility using a qualitative assay. Cell patches from a single colony were induced for pGTy1 expression by replica plating from SC-Ura-Trp medium to SC-Ura-Trp medium plus 2% galactose for 2 days at 22°C. To detect Ty1his3-AI mobility, galactose-induced cells were replica plated to SC-Ura-His medium. Below is an immunoblot assay using total cell extracts from the same strains and the p18 antiserum to detect Gag-p49/p45 and p22/p18.