TABLE 1.
Yeast strains
| Strain | Genotype | Plasmid(s) | Reference or source |
|---|---|---|---|
| DG2196 | MATa his3-Δ200hisG ura3 trp1 Ty1-less Ty1his3-AI(96) | 28 | |
| DG2254 | DG2196 | pGAL/2μ-URA3 | 28 |
| DG2255 | DG2196 | pGTy1/2μ-URA3 | 28 |
| DG2411 | DG2196 | Empty/2μ-URA3 | 28 |
| DG2374 | DG2196 | pBDG1130 (pGPOLΔ/2μ-URA3) | This study |
| YAS73 | DG2196 | pBAS39 (pGPOLΔ-T399C) | This study |
| YAS69 | DG2196 | pBAS38 (pGPOLΔ-T1108C) | This study |
| YAS71 | DG2196 | pBAS35 (pGPOLΔ-A1123G) | This study |
| YAS72 | DG2196 | pBAS36 (pGPOLΔ-A1296G) | This study |
| YAS70 | DG2196 | pBAS34 (pGPOLΔ-ΔA1456) | This study |
| YAS74 | DG2196 | pBAS43 (pGPOLΔ-Δ238-281) | This study |
| YAS75 | DG2196 | pBAS44 (pGPOLΔ-Δ238-353) | This study |
| JM321 | DG2196 | pBJM79 (pGPOLΔ-ΔC1071) | This study |
| JM320 | DG2196 | pBJM78 (pGPOLΔ-+A1303) | This study |
| DG2511 | DG2196 plus 12 Ty1 | This study | |
| DG3856 | DG2196 | pBDG1595 (pGPOLΔ-GCG1GCG2) | This study |
| DG2512 | DG2196 plus 9 Ty1 | This study | |
| DG3798 | DG2196 plus 7 Ty1-A1123G | This study | |
| DG1768 | MATα his3-Δ200hisG ura3 Ty1-less | 28 | |
| DG2533 | DG1768, Ty1–4253his3-AI | 3 | |
| DG2634 | DG1768, Ty1–4253his3-AI, plus 37 Ty1 | 31 | |
| YEM515 | DG2634, spt3-ΔkanMX4 | E. Matsuda | |
| GRF167 | MATα ura3-167 his3-Δ200 | 15 | |
| DG789 | GRF167, spt3-101 | 50 | |
| BY4742 | MATα his3-Δ1 leu2-Δ0 lys2-Δ0 ura3-Δ0 | 96 | |
| DG2247 | BY4742, spt3-ΔkanMX4 | 31 | |
| MAC103 | BY4742, xrn1/kem1-ΔkanMX4 | 7 | |
| DG3582 | DG1768, trp1 | This study | |
| DG3753 | DG3582 | pGAL/Cen-URA3, pBDG1534 (pGTy1his3-AI/Cen-TRP1) | This study |
| DG3751 | DG3582 | pBDG1354 (pGAL:1042-5889/Cen-URA3), pBDG1534 | This study |
| DG3739 | DG3582 | pGAL-Yes2/2μ-URA3, pBDG1534 | This study |
| DG3774 | DG3582 | pBDG1565 (pGAL-Yes2:1038-1616), pBDG1534 | This study |
| DG3784 | DG3582 | pBDG1565, empty/Cen-TRP1 | This study |
| JM367 | DG3582 | pBDG1568 (pGAL-Yes2:1038-V5-1616), pBDG1534 | This study |
| DG3791 | DG3582 | pBDG1571 (pGAL-Yes2:1038-1496), pBDG1534 | This study |
| JM399 | DG3582 | pBJM90 (pGAL-Yes2:1038-1616Gag*PR), pBDG1534 | This study |
| DG3808 | BY4742 | pEG(KT): pGAL:GST/2μ-URA3 | This study |
| DG3809 | BY4742 | pBDG1576 (pGAL:GST-1038-1496) | This study |
| DG3810 | DG3582 | pBDG1576 | This study |