TABLE 2.
Ty1his3-AI mobility
| Group | Strain | Relevant genotype | Ty1his3-AI mobility, 10−6 (SD) | Fold decreasea |
|---|---|---|---|---|
| A | DG2254 | Ty1-less Ty1his3-AI pGAL/2μ | 220 (69) | 1 |
| DG2374 | pGPOLΔ | 7 (1.8) | 31 | |
| YAS73 | pGPOLΔ-T399C (Gag: Ser36Pro) | 24 (5.7) | 9.2 | |
| YAS69 | pGPOLΔ-T1108C (Gag: Leu272Pro) | 100 (27) | 2.2 | |
| YAS71 | pGPOLΔ-A1123G (Gag: Tyr277Cys) | 82 (11) | 2.7 | |
| YAS72 | pGPOLΔ-A1296G (Gag: Thr335Ala) | 42 (3.4) | 5.2 | |
| YAS70 | pGPOLΔ-ΔA1456 | 31 (9.7) | 7 | |
| B | DG2196 | Ty1-less Ty1his3-AI | 120 (14) | 1 |
| DG2511 | Plus 12 Ty1 | 3.6 (0.63) | 33 | |
| DG3798 | Plus 7 Ty1A1123G | 570 (150) | 4.8↑ | |
| C-1 | DG2411 | Ty1-less Ty1his3-AI empty/2μ | 140 (30) | 1 |
| DG2374 | pGPOLΔ | 2 (0.36) | 74 | |
| JM321 | pGPOLΔ-ΔC1071 | 61 (39) | 2.3 | |
| JM320 | pGPOLΔ-+A1303 | 58 (6.2) | 2.4 | |
| C-2 | JM321 | pGPOLΔ-ΔC1071 | 65 (22) | 2.1 |
| DG3856 | pGPOLΔ-GCG1GCG2 | 137 (23) | 1 | |
| D | DG3753 | Ty1-less pGTy1his3-AI/Cen pGAL/Cen | 21,000 (2,600) | 1 |
| DG3751 | pGAL:1042-5889 | 37 (8.8) | 570 | |
| E | DG3739 | Ty1-less pGTy1his3-AI/Cen pYES2/2μ | 60,000 (4,700) | 1 |
| DG3774 | pYES2: 1038-1616 | 1.7 (0.57) | 35,000 | |
| JM367 | pYES2: 1038-V5-1616 | 2 (0.65) | 32,000 |
a
Fold changes represent decreases, except where indicated by an upward arrow.