. 2015 Jan 21;89(7):3888–3909. doi: 10.1128/JVI.03597-14

TABLE 1.

Characteristics of ORFs

ORF	Function	MneRV2,^a size (aa)	RRV,^b size (aa)	% identity	KSHV,^c size (aa)	% identity
N1	ITAM-containing signal-transducing membrane protein; KSHV K1	432	423	53.0	289	16.6
2	Dihydrofolate reductase (vDHFR)	185	188	66.5	210^d	47.0
4	Complement control protein; membrane protein; KSHV KCP	381	395	76.6	550	67.7
6	Single-strand DNA binding protein (ssDBP); UL29	1,132	1,132	94.1	1,133	37.6
7	Subunit of terminase; UL28	684	686	86.4	695	51.6
8	Glycoprotein B; UL27	826	829	91.0	845	65.7
9	DNA polymerase; UL30	1,013	1,014	93.7	1,012	66.8
10	Derived from dUTPase; CMV^k UL82/83/84	416	416	81.0	418	32.9
11	Derived from dUTPase; CMV UL82/83/84	410	409	86.6	407	31.0
N2	vIL-6; KSHV K2	207	207	82.1	204	14.5
-^e	(Position of vDHFR in KSHV)	-	-	-	210
-	(E3 ubiquitin ligase membrane protein; KSHV K3; MIR1; ORF12)	-	-	-	333
70	Thymidylate synthase (vTS)	333	333	93.1	337	65.8
N4	Interleukin 8-like CC chemokine; KSHV K4; vMIP-II; vCCL-2	120	118	75.0	94	23.3
-	(Interleukin 8-like CC chemokine; KSHV K4.1; vMIP-Iβ; vCCL-3)	-	-	-	114
-	(Contains hydrophobic domains; KSHV K4.2)	-	-	-	182
-	(E3 ubiquitin ligase membrane protein; KSHV K5; MIR2)	-	-	-	256
-	(Interleukin 8-like CC chemokine; KSHV K6; vMIP-I; vCCL-1)	-	-	-	95
N7	Related to KSHV K7; RFHVMn RF7	116	***^f	***^f	126	7.8
16	BCL-2-like inhibitor of apoptosis; vBCL-2	184	187	90.2	175	44.6
17	Minor capsid scaffold protein; UL26	537	536	82.7	553	58.3
17.5	Major capsid scaffold protein; UL26.5	292	291	77.7	288	30.0
18	Related to CMV UL79	299	257	73.6	257	56.0
19	Putative portal-capping protein; UL25	547	547	84.3	549	51.7
20	Nuclear protein; UL24	350	350	83.1	320	33.4
21	Thymidine kinase; UL23	556	557	87.0	580	41.4
22	Glycoprotein H; UL22	726	726	81.8	730	38.0
23	Tegument protein; UL21	419	402	80.2	404	45.6
24	Related to CMV UL87	733	732	85.9	752	58.5
25	Major capsid protein; UL19	1,378	1,378	95.6	1,376	72.3
26	Capsid triplex protein; UL18	305	305	93.8	305	64.3
27	Related to EBV BDLF2	273	269	76.9	290	27.1
28	Membrane protein; related to KSHV ORF28	91	91	59.3	102	26.4
29B	Putative ATPase subunit of terminase; UL15; unspliced	348	348	94.3	351	65.5
29	Putative ATPase subunit of terminase; UL15; 29A, 29B spliced	681	681	91.9	687	64.4
29A	Putative ATPase subunit of terminase; UL15; unspliced	327	327	86.9	312	60.3
30	Related to CMV UL91	76	76	72.4	77	44.7
31	Related to CMV UL92	217	217	86.2	224	41.9
32	Tegument protein; DNA packaging; UL17	464	464	80.8	454	40.1
33	Tegument protein; UL16	336	336	85.1	312	39.3
34	Related to CMV UL95	328	327	82.3	327	47.9
35	Tegument protein; UL14	149	149	90.6	151	35.6
36	Serine-threonine protein kinase; UL13	435	435	90.3	444	45.1
37	SOX; host shutoff; DNase; UL12	480	472	92.7	486	63.8
38	Alkaline exonuclease; UL11	70	69	75.7	61	44.3
39	Glycoprotein M; UL10	378	378	95.0	399	60.1
40A	Subunit of helicase-primase complex; UL8; unspliced	460	468	80.7	457	32.0
40	Subunit of helicase-primase complex; UL8; 40A, 41 spliced	638	638	78.8	669	27.1
41	Subunit of helicase-primase complex; UL8; unspliced	203	203	75.4	205	24.1
42	Tegument protein; UL7	272	272	90.8	278	44.1
43	Portal protein; UL6	581	576	93.3	605	61.8
44	Helicase subunit of helicase-primase complex; UL5	790	790	93.5	788	66.7
45	Inhibition of IRF-7; virion phosphoprotein;	304	353	67.4	407	22.4
46	Uracil-DNA glycosylase; UL2	255	255^g	82.0	255	61.2
47	Glycoprotein gL; UL1	163	163	81.0	167	28.2
48	Unknown function; related to BRRF2 EBV	389	389	82.5	402	26.0
49	Unknown function; related to BRRF1 EBV	301	301	94.0	302	53.2
50	RTA; transactivator; related to BRLF1 EBV; exons 1 and 2 spliced	575	577	83.9	691	37.7
N8γ	bZIP transcription factor; KSHV K8γ; exon 1 unspliced	162	177	50.9	239	13.0
N8α	bZIP transcription factor; KSHV K8α; exons 1, 2, and 3 spliced	222	234	62.2	237	19.6
N8.1γ	Glycoprotein; ORF51; KSHV K8.1γ; exon 1 unspliced	195	230	56.8	197	12.6
N8.1α	Glycoprotein; ORF51; KSHV K8.1α; exons 1 and 2 spliced	274	275	61.8	228	11.8
52	Related to BLRF2 EBV	138	139	87.0	131	43.5
53	Glycoprotein N; UL49A	96	104	87.5	110	47.9
54	Deoxyuridine triphosphatase; UL50	290	290	90.3	318	40.7
55	Tegument protein; UL51	210	210	95.7	227	56.2
56	Primase subunit of helicase-primase complex; UL52	839	828	88.4	843	51.6
57	Posttranscriptional regulator; UL54; exons 1 and 2 spliced	447	446	87.9	455	38.2
57A	Posttranscriptional regulator; UL54; unspliced	443	442	87.1	-	-
N9-1	Related to KSHV vIRF-1/K9; unspliced	414	415	69.8	449^h	19.3
N9-2	Related to KSHV vIRF-1/K9; unspliced	413	415	70.7	449^h	17.2
N9-3	Related to KSHV vIRF-1/K9; unspliced	351	351	82.6	449^h	20.5
N9-4	Related to KSHV vIRF-1/K9; unspliced	322	361	74.8	449^h	19.6
N9-5	Homolog of N9-1; related to KSHV vIRF-1/K9; unspliced	386	385	81.3	449^h	19.9
N9-6	Homolog of N9-2; related to KSHV vIRF-1/K9; unspliced	394	390	77.4	449^h	20.6
N9-7	Homolog of N9-3; related to KSHV vIRF-1/K9; unspliced	355	355	81.7	449^h	19.2
N9-8	Homolog of N9-4; related to KSHV vIRF-1/K9; unspliced	364	364	80.5	449^h	14.6
-	(KSHV vIRF-4/K10; spliced)	-	-	-	911
-	(KSHV vIRF-3/K10.5; spliced)	-	-	-	566
-	(KSHV vIRF-2/K11; spliced)	-	-	-	680
58	Multiple transmembrane protein; UL43	360	360	88.9	357	36.1
59	DNA polymerase processivity factor; UL42	394	394	88.3	396	51.8
60	Ribonucleotide reductase small subunit; UL40	314	304	91.4	305	67.8
61	Ribonucleotide reductase large subunit; UL39	790	788	91.0	792	61.5
62	Capsid triplex protein; UL38	331	331	92.7	331	57.1
63	Tegument protein; UL37	938	939	83.5	927	41.3
64	Large tegument protein; UL36	2,554	2,548	74.5	2,635	17.3
65	Small capsid protein on hexon tips; UL35	169	169	82.8	170	36.1
66	BFRF2 EBV homolog; related to CMV UL49	443	448	77.4	429	43.3
67	Capsid docking protein on nuclear lamina; UL34	268	268ⁱ	79.9	271	58.2
67A	DNA packaging; UL33	89	86	82.0	80	57.3
68	Nuclear localization of capsids and DNA packaging; UL32	457	457	82.1	545	50.3
69	Egress of capsids from nucleus; UL31	297	297	89.9	225	48.8
N12	Homolog of KSHV K12; Kaposin A	109	89	32.2	60	11.9
N13	FLIP-like inhibitor of apoptosis; vFLIP; KSHV K13; ORF71	174	174	81.0	188	33.9
72	Cyclin D-like protein; KSHV vCyc	250	254	90.4	257	35.2
73	Latency-associated nuclear antigen; KSHV LANA	438	448	59.0	1,162	16.2
N14	vOX2; contains two Ig domains; KSHV K14	254	253	83.9	348	31.5
74	vGPCR; G-protein-coupled receptor; similar to IL-8 receptor	437	342	66.6	342	32.5
75	Tegument protein; related to EBV FGARAT	1,296	1,298	84.7	1,296	43.5
N15	Signal-transducing membrane protein; K15; EBV LAMP; spliced	529	***^j	***^j	490	13.0

MneRV2 genome (KP265674).

RRV26-95 (AF10726).

KSHV genome (NC_009333).

The KSHV vDHFR is not located at this position within the KSHV genome.

KSHV-specific ORFs missing in RFHVMn and/or RRV are indicated in parentheses, and their relative positions within the macaque viral genomes are indicated by a hyphen.

The homologous ORF in RRV17577 and RRV26-95 is disrupted.

The 255-aa RRV26-95 ORF46 sequence is corrected from the published genomic sequence, which encoded an erroneous 230-aa ORF due to two single-base-pair insertion sequencing errors at bp 62577 (extra T) and bp 62668 (extra A), as described in the text.

KSHV K9.

ⁱ

The 268-aa RRV26-95 ORF67 sequence is corrected from the published genomic sequence, which encoded an erroneous 222-aa ORF due to a base pair insertion sequencing error at bp 107619 (extra C), as described in the text. An additional sequencing error was identified in the RRV17577 ORF67 at bp 109544 (missing C), by sequence comparison. The corrected RRV17577 ORF67 sequence was 268 aa and 100% identical to the corrected RRV26-95 sequence.

R15 gene in RRV26-95 is disrupted as indicated in the text. The homologous gene in RRV 17577 (532 aa) has 61.1% identity with N15.

CMV, cytomegalovirus.