TABLE 4.
Effects of mutations in the HA of A/Indonesia/CDC669/06 virus on the reactivity to h8A8a in HI and virus neutralization assays
| Virus | Mutation type | HI titerb |
VNc titerb |
||
|---|---|---|---|---|---|
| Befored | Aftere | Before | After | ||
| CDC669 | None | 7 | 7 | 8 | 8 |
| K218Q mutant | Conformational epitope | 7 | <f | 8 | < |
| S223R mutant | Conformational epitope | 7 | < | 8 | 8 |
| S223R R189K mutant | Conformational epitope | 7 | < | 8 | 8 |
| N244A mutant | Linear epitope | 7 | 7 | 8 | 8 |
| I256A mutant | Linear epitope | 7 | 7 | 8 | 8 |
a
The h8A8 concentration used was 80 μg/ml.
b
HI and VN titers of h8A8 against the strains shown are in log2 units.
c
VN, virus neutralization.
d
Before, before mutation.
e
After, after mutation.
f
<, the titer is less than 4-fold (2 log2).