Skip to main content
PMC home page
Nature Portfolio logoLink to Nature Portfolio
. 2015 Apr 13;6:6794. doi: 10.1038/ncomms7794

Regulation of systemic energy homeostasis by serotonin in adipose tissues

Chang-Myung Oh 1,*, Jun Namkung 1,2,*, Younghoon Go 3,*, Ko Eun Shong 1, Kyuho Kim 1, Hyeongseok Kim 1, Bo-Yoon Park 3,4, Ho Won Lee 5, Yong Hyun Jeon 5,6, Junghan Song 7, Minho Shong 8, Vijay K Yadav 9, Gerard Karsenty 10, Shingo Kajimura 11, In-Kyu Lee 3,4,a, Sangkyu Park 1,12,b, Hail Kim 1,c
PMCID: PMC4403443  PMID: 25864946

Abstract

Central serotonin (5-HT) is an anorexigenic neurotransmitter in the brain. However, accumulating evidence suggests peripheral 5-HT may affect organismal energy homeostasis. Here we show 5-HT regulates white and brown adipose tissue function. Pharmacological inhibition of 5-HT synthesis leads to inhibition of lipogenesis in epididymal white adipose tissue (WAT), induction of browning in inguinal WAT and activation of adaptive thermogenesis in brown adipose tissue (BAT). Mice with inducible Tph1 KO in adipose tissues exhibit a similar phenotype as mice in which 5-HT synthesis is inhibited pharmacologically, suggesting 5-HT has localized effects on adipose tissues. In addition, Htr3a KO mice exhibit increased energy expenditure and reduced weight gain when fed a high-fat diet. Treatment with an Htr2a antagonist reduces lipid accumulation in 3T3-L1 adipocytes. These data suggest important roles for adipocyte-derived 5-HT in controlling energy homeostasis.

Inline graphicThe neurotransmitter serotonin has both central and peripheral effects. Here, the authors show that adipocyte-derived serotonin regulates organismal energy homeostasis in mice by acting on adipocyte serotonin receptors on fat cells, which regulates lipolysis and thermogenesis in white and brown fat tissue.

5-Hydroxytryptamine (5-HT, serotonin) is a monoamine that modulates central and peripheral functions. It is primarily found in the gastrointestinal tract, platelets, pineal gland and the central nervous system. 5-HT is synthesized from the essential amino acid tryptophan by the sequential actions of tryptophan hydroxylase (Tph) and aromatic amino acid decarboxylase. Hydroxylation of tryptophan is the initial and rate-limiting step in the synthesis of 5-HT. There are two isoforms of Tph: Tph1 and Tph2. Tph1 is primarily expressed in peripheral tissues, whereas Tph2 is exclusively expressed in neuronal tissues including the central nervous system and enteric neurons1. 5-HT commonly acts locally in neural and paracrine circuits, and it has a variable function depending on the tissue2. The action of released 5-HT is terminated by uptake into cells through 5-HT transporter (SERT)3.

As 5-HT cannot cross the blood–brain barrier, central and peripheral 5-HT systems are functionally separated. Almost 90% of body 5-HT is synthesized peripherally in the gastrointestinal tract and stored in platelets. Small amount of 5-HT is also present in other peripheral tissues4. Once released, 5-HT exerts its biological action by binding to 5-HT receptor (Htr). More than 14 Htrs have been identified and they are G-protein-coupled receptor except for Htr3, which is a ligand-gated cation channel.

Central 5-HT functions as an anorexigenic neurotransmitter by activating the Htr2c in the brain5,6,7,8. Direct intracranial injection of p-chlorophenylalanine (PCPA), a Tph inhibitor, into the ventricle induced marked hyperphagia and obesity9. However, body weight was reduced in Tph1 and Tph2 knockout (KO) mice10. Mice with a SERT-null mutation (Slc6a4 KO) are expected to be slim due to the increased 5-HT activity, but these mice exhibit an obese phenotype11. The enhancement of 5-HT activity using a selective SERT inhibitor was associated with weight loss, but the effect was transient and restoration occurred during maintenance period12. These discordant results suggest that peripheral 5-HT might have opposite functions to central 5-HT in the regulation of energy homeostasis.

Here we show that 5-HT has a functional role in adipose tissues. We inhibited 5-HT synthesis in mice genetically by inducing Tph1 KO in adipose tissue and pharmacologically by administrating the systemic Tph inhibitor PCPA13 and the peripheral Tph inhibitor LP-533401 (ref. 14). Under high-fat diet (HFD) condition, the inhibition of 5-HT synthesis reduced body weight gain, improved glucose tolerance, increased thermogenic activity in brown adipose tissue (BAT) and decreased lipogenesis in white adipose tissue (WAT). We also show that 5-HT inhibited thermogenesis through Htr3 in BAT and increased lipogenesis through Htr2a in WAT. Our data indicate that adipocyte-derived 5-HT plays important roles in controlling energy homeostasis and might be a therapeutic target for obesity and metabolic disease.

Results

Reduced weight gain by inhibiting 5-HT synthesis

We hypothesized that if peripheral 5-HT has opposite effects to central 5-HT in the regulation of body weight, long-term systemic inhibition of 5-HT synthesis may reduce body weight or the degree of weight gain by an HFD. In this regard, mice were fed an HFD and administered PCPA by intraperitoneal injection for 12 weeks from 11 weeks of age. PCPA-treated mice ate more food than control mice during the first week of HFD, but their food intake became comparable to control mice from the second week throughout the HFD period. These changes of eating patterns matched well with previous reports9. As a result of the systemic inhibition of 5-HT synthesis, PCPA-treated mice exhibited decreased body weight gain on an HFD (Fig. 1a) and their visceral fat mass was reduced (Fig. 1b), although they showed similar body weight on a standard chow diet (SCD).

Figure 1. PCPA protects against diet-induced obesity.

Figure 1

Open in a new tab

(a) Growth curves of vehicle- or PCPA-treated mice fed an SCD or HFD. n=4 mice per group. *P<0.05 versus HFD+PCPA by Student's t-test. (b) Gross images of vehicle- or PCPA-treated mice after 10 weeks of HFD feeding. (c) Intraperitoneal glucose tolerance test (IPGTT) after fasting for 16 h. n=3 mice per group. *P<0.05 versus HFD by Student's t-test. (d) Intraperitoneal insulin tolerance test (IPITT) after 4 h fasting. n=3 mice per group. *P<0.05 versus HFD by Student's t-test. (e) The metabolic rates of vehicle- or PCPA-treated mice after 6 weeks of HFD feeding. The metabolic parameters were measured using an 8-chamber Oxymax system. Mice were acclimatized to cages for 24 h and data were collected for an additional 48 h. n=4 mice per group. *P<0.05 versus vehicle by Student's t-test. All data are presented as the mean±s.e.

The severe loss of visceral fat mass after 12 weeks of PCPA treatment concerned that massive destruction of adipose tissue might cause lipodystrophy. However, we could find small fat cells with normal structure in WAT (Supplementary Fig. 1a). PCPA treatment improved glucose tolerance and insulin sensitivity in the HFD-fed mice (Fig. 1c,d). The serum levels of total cholesterol, free fatty acid and leptin were decreased in accordance with the reduction of WAT in the PCPA-treated mice (Supplementary Fig. 1b). Intriguingly, serum adiponectin levels were also decreased in PCPA-treated mice (Supplementary Fig. 1b). As 5-HT is known to decrease adiponectin expression in 3T3-L1 adipocytes15 and adiponectin improves insulin sensitivity, PCPA treatment was expected to increase serum adiponectin levels, which the improved insulin sensitivity could be attribute to. Thus, the decreased serum adiponectin in PCPA-treated mice is more likely to be an indirect reflection of reduced fat mass rather than the direct downregulation of adiponectin expression by inhibiting 5-HT synthesis in adipose tissues.

To investigate the mechanism of reduced weight gain following systemic administration of PCPA, we analysed the food intake and energy expenditure using indirect calorimetry. Under HFD condition, PCPA-treated mice showed higher oxygen consumption and heat production than control mice that could not be attributed to changes in food intake or physical activity (Fig. 1e and Supplementary Fig. 1c). However, PCPA treatment did not affect the metabolic rates of mice fed an SCD (Supplementary Fig. 1d), suggesting the requirement of metabolic stress for the positive effect of PCPA on energy expenditure. These data suggested that peripheral 5-HT affected positively on body weight control in contrast to central 5-HT.

Inhibition of 5-HT synthesis increased thermogenesis

In peripheral tissues, 5-HT is mainly produced and secreted by enterochromaffin cells in the gut and actively taken up by platelets, which store most of the body 5-HT2,16. However, gut-derived 5-HT is not associated with diet-induced obesity17, suggesting more localized effects of 5-HT in regulating energy homeostasis. It has been known that 5-HT is present in WAT and BAT, and it promotes adipogenesis in 3T3-L1 preadipocytes15,18,19. Thus, we tested 5-HT production in adipose tissues. Indeed, all the genes involved in 5-HT metabolism, except for Tph2, were expressed in adipose tissues (Supplementary Fig. 2a). Interestingly, the HFD feeding increased the Tph1 messenger RNA level in epididymal WAT (eWAT) and inguinal WAT (iWAT), and increased tissue 5-HT levels accordingly (Fig. 2a,b). These data suggested the potential role of adipocyte-derived 5-HT in the development of diet-induced obesity. Therefore, we investigated metabolic changes in adipose tissue.

Figure 2. PCPA increased metabolic activity in BAT.

Figure 2

Open in a new tab

(a) Tph1 mRNA expression in adipose tissues was assessed by quantitative reverse transcriptase–PCR (qRT–PCR) after 2 weeks of HFD feeding. n=4 mice per group. *P<0.05 versus SCD by Student's t-test. (b) Tissue 5-HT levels were assessed by LC-MS after 2 weeks of HFD feeding. n=4 mice per group. *P<0.05 and ***P<0.001 versus SCD by Student's t-test. (c) Metabolic activity of BAT of vehicle- or PCPA-treated mice after 6 weeks of HFD feeding was assessed by PET-computed tomography (CT). Representative axial PET-CT images (left panel) and quantitative comparisons of 18fluorodeoxyglucose (18F-FDG) uptake in PET-CT images (right panel). BAT (triangle) and the heart (arrow) are highlighted. *P<0.05 versus vehicle by Student's t-test. (d) Haematoxylin and eosin (H&E) staining of BAT sections from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. Scale bar, 20 μm. (e) Expression of thermogenesis-associated genes in BAT, as assessed by qRT–PCR. n=5 per group. *P<0.05 and ***P<0.001 versus SCD by Student's t-test. All data are presented as the mean±s.e. LC–MS: liquid chromatography–mass spectrophotometry.

To examine the mechanism of increased energy expenditure by PCPA treatment, we measured the metabolic activity of mouse organ by assessing 18fluorodeoxyglucose uptake, using positron emission tomography (PET)-computed tomography20. We found that the inhibition of 5-HT synthesis by PCPA significantly increased glucose uptake into BAT (Fig. 2c). Histological analysis also revealed that PCPA-treated mice showed decreased lipid droplet size and increased multilocular adipocytes in BAT (Fig. 2d). PCPA treatment increased the mRNA expression of thermogenic genes in BAT and the highest increase was observed in the Dio2 mRNA level (Fig. 2e and Supplementary Fig. 2b). In addition, the number and size of the mitochondria and the density of the cristae were increased in the BAT of PCPA-treated mice (Supplementary Fig. 2c). These data suggested that inhibition of 5-HT synthesis increased the thermogenic activity of BAT.

In eWAT, PCPA administration led to a decrease in adipocyte size with normal cellular structures (Fig. 3a,b). The expression of lipogenic genes was decreased in PCPA-treated mice compared with control mice (Fig. 3c and Supplementary Fig. 3a). In the iWAT of PCPA-treated mice, the adipocyte size was decreased and brown adipocyte-like cells expressing Ucp1 were observed (Fig. 3d-f), indicating the browning of iWAT21. In agreement with the Ucp1 immunostaining, Ucp1 and Dio2 mRNA levels were increased in iWAT following PCPA treatment (Fig. 3g and Supplementary Fig. 3b). These results suggested that 5-HT might play a role in lipogenesis and thermogenesis in WAT.

Figure 3. PCPA decreased lipogenesis in eWAT and induces brown fat-like changes in iWAT.

Figure 3

Open in a new tab

(a) Representative haematoxylin and eosin (H&E) images of eWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (b) Average adipocyte sizes of eWAT were measured from H&E images using ImageJ software. n=5 mice per group. *P<0.05 and versus vehicle by Student's t-test. ***P<0.001 versus SCD+vehicle and HFD+PCPA by two-way analysis of variance (ANOVA). (c) Expression of genes associated with lipogenesis in eWAT, as assessed by quantitative reverse transcriptase–PCR (qRT–PCR). n=4 mice per group. *P<0.05 and ***P<0.001 versus vehicle by Student's t-test. (d) Representative H&E images of iWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (e) Average adipocyte sizes of iWAT were measured from H&E images using ImageJ software. n=5 mice per group. ***P<0.001 versus SCD+vehicle and HFD+PCPA by two-way ANOVA. (f) Immunohistochemical staining for Ucp1 in iWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (g) Ucp1 gene expression in iWAT was assessed via qRT–PCR. n=4 mice per group. ***P<0.001 versus vehicle by Student's t-test. All data are presented as the mean±s.e. Scale bar, 20 μm.

Peripheral Tph inhibitor prevents HFD-induced obesity

To exclude the possibility that anti-obesity effects of PCPA might be related to the inhibition of 5-HT synthesis in the brain, we have tested peripheral Tph inhibitior, LP-533401, which cannot cross the blood–brain barrier14. Mice treated with LP-533401 showed reduced weight gain and improved glucose tolerance compared with control mice under an HFD (Fig. 4a,b). The BAT of LP-533401-treated mice displayed similar histological changes to those observed in PCPA-treated mice after an HFD feeding (Fig. 4c). The BAT of LP-533401-treated mice also showed increased thermogenic gene expressions (Fig. 4d). Taken together, our data suggested that the inhibition of peripheral Tph1 increased energy expenditure by increasing thermogenic activity of BAT and iWAT.

Figure 4. LP-533401 protects against diet-induced obesity and increased BAT activity.

Figure 4

Open in a new tab

Mice were treated orally with vehicle or LP-533401 (30 mg kg−1) and fed an SCD or HFD for 14 weeks from 8 weeks of age. (a) Growth curves of vehicle- or LP-533401-treated mice fed an HFD. n=5 mice per group. *P<0.05, **P<0.01 and ***P<0.001 versus HFD+LP-533401 by Student's t-test. (b) Intraperitoneal glucose tolerance test (IPGTT) after 16 h fasting n=4 mice per group. *P<0.05 and **P<0.01 versus HFD+LP-533401 by Student's t-test. (c) Representative haematoxylin and eosin (H&E) images of BAT of vehicle- or LP-533401-treated mice. Scale bar, 20 μm. (d) Expression of thermogenesis-related genes in BAT was assessed by quantitative reverse transcriptase–PCR (qRT–PCR). n=4 mice per group. *P<0.05 versus vehicle by Student's t-test. All data are presented as the mean±s.e.

Cell autonomous function of 5-HT in adipose tissues

To test the cell autonomous function of 5-HT in adipose tissues, we isolated the stromal vascular fraction (SVF) from BAT of adipocyte-specific Tph1 KO (Adipoq-Cre+/−/Tph1floxlflox, Tph1 FKO) mice and differentiated into mature brown adipocytes. After 8 days of culture in differentiation medium, Ucp1 expression was upregulated in Tph1-null brown adipocytes, which was abrogated by 5-HT treatment (Fig. 5a). Furthermore, the increase in Ucp1 mRNA expression by β3 adrenergic receptor (β3AR) stimulation was significantly augmented in the Tph1-null brown adipocytes (Fig. 5a). These data demonstrated the inhibitory role of 5-HT in thermogenic activity of BAT22.

Figure 5. Cell autonomous function of 5-HT in adipocytes.

Figure 5

Open in a new tab

(a) Ucp1 mRNA levels were analysed by quantitative reverse transcriptase–PCR (qRT–PCR) using SVF from the BAT of Tph1 FKO mice or their WT littermates. Cells were cultured in BAT differentiation medium for 8 days and then treated with 5-HT (10 μM) or β3AR agonist (CL 316243, 100 nM). n=3 mice per group. *P<0.05 for Tph1 FKO versus WT control and Tph1 FKO-5-HT by two-way analysis of variance (ANOVA). ***P<0.001 versus WT by Student's t-test. (b) Serum 5-HT level of WT and Tph1 AFKO mice after 6 weeks of HFD feeding. n=4 mice per group. (c) Representative haematoxylin and eosin (H&E) images of eWAT, iWAT and BAT from Tph1 AFKO mice and their WT littermates fed an SCD. Data are presented as the mean±s.e. Scale bar, 20 μm.

To investigate the role of 5-HT in mature adipocytes, we generated inducible Tph1 KO (aP2-CreERT2+/−/Tph1floxlflox, Tph1 AFKO) mice and induced Tph1 KO in adipose tissues at 6 weeks of age by injecting tamoxifen intraperitoneally. Tph1 AFKO mice looked grossly normal, maintained normal serum 5-HT levels (Fig. 5b) and no histological difference was observed in their adipose tissues (Fig. 5c). However, Tph1 AFKO mice showed reduced weight gain, improved glucose tolerance and insulin sensitivity compared with wild-type (WT) littermates after 6 weeks of HFD feeding (Fig. 6a–c). HFD-fed Tph1 AFKO mice showed similar histological changes in adipose tissues as the PCPA-treated mice, reduced adipocyte size in both eWAT and iWAT (Fig. 6d,e), increased Ucp1 expression in iWAT (Fig. 6f) and increased multilocular adipocytes in BAT (Fig. 6g). These phenotypes of Tph1 AFKO mice suggested the important role of adipocyte-derived 5-HT in the regulation of systemic energy homeostasis.

Figure 6. Tph1 AFKO mice are resistant to diet-induced obesity.

Figure 6

Open in a new tab

(a) Growth curves of Tph1 AFKO mice and their WT littermates fed an HFD. n=4 mice per group. *P<0.05, **P<0.01 and ***P<0.001 versus WT by Student's t-test. (b) Intraperitoneal glucose tolerance test (IPGTT) in HFD-fed Tph1 AFKO mice and their WT littermates after 16h fasting. n=4 mice per group. *P<0.05 versus WT by Student's t-test. (c) Intraperitoneal insulin tolerance test (IPITT) in HFD-fed Tph1 AFKO mice and their WT littermates after 4h fasting. n=4 mice per group. **P<0.01 versus WT by Student's t-test. (d) Representative hematoxylin and eosin (H&E) images of WAT of Tph1 AFKO mice and their WT littermates after 6 weeks of HFD feeding. (e) Average adipocyte sizes of eWAT and iWAT were measured from H&E images using ImageJ software. n=4 mice per group. *P<0.05 versus WT by Student's t-test. (f) Immunohistochemical staining for Ucp1 in iWAT from Tph1 AFKO mice and their WT littermates after 6 weeks of HFD feeding. (g) Representative H&E images of BAT of Tph1 AFKO mice and their WT littermates after 6 weeks of HFD feeding. All data are presented as the mean±s.e. Scale bar, 20μm.

5-HT regulates thermogenesis via Htr3

As the anti-obesity effects of PCPA were attributed to the potentiation of adaptive thermogenesis in BAT, we attempted to identify the Htr responsible for the activation of BAT. Among Htrs in BAT (Supplementary Fig. 2a), we focused on Htr3, which is a heteropentamer of Htr3a and Htr3b, and acts as a functional 5-HT-gated cation channel23,24. Previously, we reported that Htr3 regulates glucose-stimulated insulin secretion in pancreatic islets25. While we were studying the effects of Htr3 in insulin secretion, we noticed that HFD-fed Htr3a KO (Htr3a−/−) mice showed improved insulin sensitivity and reduced weight gain after 10 weeks of age25,26. These findings prompted us to test the role of Htr3 in adaptive thermogenesis.

Htr3a KO mice were fed an HFD for 6 weeks from 10 weeks of age and analysed their metabolic phenotypes (Supplementary Fig. 4a). As shown in Fig. 7, Htr3a KO mice were resistant to HFD-induced obesity (Fig. 7a,b). However, glucose tolerance was not improved in Htr3a KO mice, despite the improved insulin sensitivity (Fig. 7c,d). Defective insulin secretion in Htr3a KO mice can explain the discrepancy between glucose tolerance and insulin sensitivity26. As expected, Htr3a KO mice exhibited increased oxygen consumption and heat production compared with their WT littermates (Fig. 7e and Supplementary Fig. 4b). HFD-fed Htr3a KO mice did not exhibit enlarged unilocular lipid droplets in the BAT, which were observed in the BAT of their WT littermates after HFD feeding (Fig. 8a). Thermogenic gene expressions also increased in BAT of Htr3a KO mice (Fig. 8b). Furthermore, mitochondrial biogenesis was increased in BAT of Htr3a KO mice (Fig. 8c,d). These data suggested that the metabolic and histological changes observed in HFD-fed Tph1 AFKO mice could be attributed to the reduced Htr3 activity in BAT. However, WAT of Htr3a KO mice did not exhibit similar changes to Tph1 AFKO after HFD, suggesting the more selective effects of Htr3 in BAT (Figs 7b and 8e).

Figure 7. Htr3a KO mice are resistant to diet-induced obesity.

Figure 7

Open in a new tab

(a) Growth curves of Htr3a KO mice and their WT littermates fed an SCD or HFD. n=4 mice per group. **P<0.01 versus WT, HFD by Student's t-test. (b) Gross images of Htr3a KO mice fed an SCD or HFD for 6 weeks beginning at 8 weeks of age. (c) Intraperitoneal glucose tolerance test (IPGTT) after 16 h fasting. n=4 mice per group. (d) Intraperitoneal insulin tolerance test (IPITT) after 4 h fasting. n=4 mice per group. **P<0.01 and ***P<0.001 versus WT, HFD by Student's t-test. (e) The metabolic rates of Htr3a KO mice and their WT littermates after 6 weeks of HFD feeding. n=4 mice per group. ***P<0.001 versus WT by Student's t-test. All data are presented as the mean±s.e.

Figure 8. Htr3a KO mice shows increased BAT activity.

Figure 8

Open in a new tab

(a) Representative haematoxylin and eosin (H&E) images of BAT of Htr3a KO mice and their WT littermates after 6 weeks of HFD feeding. Scale bar, 20 μm. (b) Expression of thermogenesis-related genes in BAT was assessed by quantitative reverse transcriptase–PCR (qRT–PCR). n=4 per group. *P<0.05 versus WT by Student's t-test. (c) Representative transmission electron microscopic (TEM) images of mitochondria in BAT from Htr3a KO mice and WT littermates after 6 weeks of HFD feeding. Scale bar, 1 μm. (d) Mitochondrial gene expressions in BAT isolated from Htr3a KO mice and their WT littermates after 6 weeks of HFD feeding. The mRNA expressions of mitochondrial biogenesis-associated genes were assessed using qRT–PCR. n=4 per group. *P<0.05 versus WT by Student's t-test. (e) Representative H&E images of eWAT and iWAT from Htr3a KO mice and their WT littermates after 6 weeks of HFD feeding. Scale bar, 20 μm. All data are presented as the mean±s.e.

To know the localized actions of Htr3 in BAT, we explored the effects of an Htr3 antagonist on immortalized brown adipocytes (IBA). After differentiation, cells were treated with an Htr3 antagonist (ondansetron) or an Htr3 agonist (1-(m-chlorophenyl)-biguanide, m-CPBG), and thermogenic response to β3AR stimulation was tested. Ondansetron and m-CPBG did not have significant effects on IBA in the absence of the β3AR agonist (Fig. 9a,b). However, ondansetron increased cyclic AMP production and phosphorylation of hormone-sensitive lipase (Hsl) and protein kinase A substrate in the presence of the β3AR agonist (Fig. 9a,b and Supplementary Fig. 4c). Ondansetron also increased the mRNA expression of thermogenic genes, such as Ucp1 and Ppargc1a, in IBA (Fig. 9c). Conversely, m-CPBG decreased Ucp1 mRNA in IBA (Fig. 9d). To examine whether blocking of Htr3 increases the energy metabolism of brown adipocytes, we measured the oxygen consumption rate (OCR) of IBA using XF analyser. Ondansetron increased the OCR synergistically with the β3AR agonist (Fig. 9e,f). Ex vivo experiment using primary BAT from Htr3a KO mice showed similar results. Primary brown adipocytes lacking Htr3 showed higher Ucp1 expression and higher sensitivity to β3AR stimulation (Fig. 9g). Taken together, these data indicated that 5-HT regulates thermogenesis in BAT through Htr3 in cell autonomous manner.

Figure 9. Cell autonomous effects of Htr3 on BAT activity.

Figure 9

Open in a new tab

The effects of the Htr3 agonist and antagonist, and β3AR agonist were tested in IBA and in primary BAT from Htr3a KO mice. (a,b) IBA pretreated with Ondansetron (1 μM) or m-CPBG (100 nM) were treated with vehicle or CL 316243 (1 μM) for 15 min. (a) Intracellular cAMP levels of IBA were measured by enzyme-linked immunosorbent assay (ELISA). (b) Protein kinase A (PKA) substrate, Hsl, and phosphorylation of Hsl on ser660 were assessed by western blot analysis. (c) Expression of thermogenesis-related genes in IBA was assessed by quantitative reverse transcriptase–PCR (qRT–PCR) after 2 h ondansetron (1 μM) treatment. n=4 per group. *P<0.05 and **P<0.01 versus vehicle by Student's t-test. (d) Ucp1 mRNA expression in IBA was assessed by qRT–PCR after 2 h m-CBPG (100 nM) treatment. n=4 per group. **P<0.01 versus vehicle by Student's t-test. (e) Oxygen consumption rate (OCR) of IBA was assessed using the Seahorse XF24 analyser. n=5 per group. *P<0.05 versus vehicle+CL 316243 by Student's t-test. (f) Average OCR of IBA after CL 316243 treatment. n=5 per group. *P<0.05 versus vehicle+CL 316243 by Student's t-test. (g) Ucp1 mRNA expression of primary brown fat adipocytes isolated from Htr3a KO mice and WT littermates. n=4 per group. *P<0.05 versus WT by Student's t-test. All data are presented as the mean±s.e. Ondan, ondansetron; CL, CL 316243; m-CBPG, 1-(m-chlorophenyl)-biguanide.

5-HT regulates lipogenesis via Htr2a

In contrast to the results of Tph1 inhibition genetically or pharmacologically, Htr3a KO mice maintained substantial amount of eWAT mass and no histological differences were observed in the eWAT and iWAT compared with the WT littermates. These results suggested that the effect of Htr3 inhibition is more selective in BAT, and that another mechanism could be responsible for the lipogenesis of WAT. In an attempt to identify the additional mechanism that could explain the reduction of eWAT mass caused by inhibiting Tph1 we focused on Htr2a, because recent genetic association studies have reported that HTR2A is significantly associated with obesity27,28,29. In addition, 5-HT is an adipogenic inducer of 3T3-L1 adipocytes and Htr2a expression is increased in hypertrophied 3T3-L1 adipocytes15,18. Indeed, 3T3-L1 adipocytes synthesized 5-HT during differentiation (Fig. 10a) and the expression of Htr2a gradually increased after day 8 (Fig. 10b). These results suggest that 5-HT may regulate lipogenesis in mature adipocytes through Htr2a. Therefore, we treated 3T3-L1 adipocytes with an Htr2a agonist (2,5-dimethoxy-4-iodoamphetamine, DOI) or an Htr2a antagonist (ketanserin), and assessed mRNA expression of lipogenic genes. DOI increased the mRNA levels of lipogenic genes in mature adipocytes (Fig. 10c). On the other hand, ketanserin decreased lipid accumulation (Fig. 10d). In the glycerol release assay, 5-HT and the DOI suppressed lipolysis in mature adipocytes (Fig. 10e). These data indicated that 5-HT positively regulates lipogenesis in mature adipocytes through Htr2a.

Figure 10. Htr2a regulates lipogenesis in WAT.

Figure 10

Open in a new tab

(a) The immunofluorescence staining of 5-HT in 3T3-L1 adipocytes during differentiation. Scale bar, 20 μm. (b) Htr2a mRNA expression in 3T3-L1 adipocytes during differentiation was assessed by quantitative reverse transcriptase–PCR (qRT-PCR). n=3 per group. *P<0.05 versus D0 by one-way analysis of variance (ANOVA). (c) Lipogenic gene expression in differentiated 3T3-L1 adipocytes was assessed by qRT–PCR after Htr2a agonist (100 nM DOI) treatment for 24 h. n=3 per group. ***P<0.001 versus vehicle by Student's t-test. (d) Lipid accumulation in 3T3-L1 adipocytes was quantified. Cells were stained with Oil Red O and optical density (OD) of cell extracts was quantified using a spectrophotometer. n=4 per group. *P<0.05 versus vehicle by one-way ANOVA. (e) Lipolysis was assessed by a glycerol release assay in differentiated 3T3-L1 adipocytes. n=6 per group. *P<0.05 versus vehicle and ***P<0.001 versus isoproterenol by one-way ANOVA. (f) A proposed model for the regulation of energy homeostasis by 5-HT in adipocytes. Red arrows denote activation and blue arrows denote suppression. All data are presented as the mean±s.e. Vehicle: PBS; DOI (2,5-dimethoxy-4-iodoamphetamine): Htr2a agonist; ketanserin: Htr2a antagonist.

Discussion

Although most studies on the effects of 5-HT on obesity have been focused on its central action5,6,7,8, recent studies have reported the relationship between peripheral 5-HT and obesity30,31. Genetic studies have reported that polymorphisms in human Htr genes (for example, HTR1A, HTR1Dβ and HTR2A) are associated with obesity32,33,34. A recent study using Tph1 KO mice reported that inhibition of peripheral Tph1 protects against diet-induced obesity and promotes BAT thermogenesis35. They found that inhibition of Tph1 increases the sensitivity of BAT to β3AR stimulation and its effects depend on Ucp1-mediated thermogenesis.

Most peripheral 5-HT is produced in enterochromaffin cells in the gut and stored in platelets. However, gut-specific Tph1 KO mice did not show resistance to diet-induced obesity17, which led us to focus on adipose tissue-derived 5-HT. In the present study, we demonstrated that adipocytes can produce 5-HT separately from the gut and HFD increases Tph1 mRNA expression and tissue 5-HT levels in adipose tissues. Mice with inducible Tph1 KO in adipose tissues were resistant to HFD-induced weight gain and their glycemic control was improved.

Adaptive thermogenesis was enhanced in BAT of Htr3a KO mice after HFD feeding, which indicated that 5-HT regulates thermogenesis of BAT via Htr3. Previously, we reported that Htr3 activation depolarizes the β-cell membrane, thereby increasing glucose-stimulated insulin secretion in pancreatic islets25. The membrane potential is also important in BAT activity. The activation of BAT in response to β3AR stimulation involves a transient hyperpolarization of membrane potential36, suggesting the possibility that Htr3 inhibition could enhance the responsiveness of BAT to β3AR stimulation through membrane hyperpolarization.

Although Htr3a KO mice showed reduced weight gain in HFD-induced obesity model, their WAT did not show remarkable differences in fat mass and histology compared with WAT of WT littermates (Figs 7a,b and 8e), suggesting that insulin resistance of Htr3a-null WAT was comparable to WT WAT. Thus, the improved insulin sensitivity of Htr3a KO mice is probably due to the enhanced adaptive thermogenesis in BAT. In this context, obese WAT of Htr3a KO mouse could be considered as a neutral bystander, otherwise insulin sensitivity would not be improved in HFD-fed Htr3a KO mice. In addition, the inhibition of 5-HT synthesis under HFD resulted in the decreased lipogenesis in WAT and the increased thermogenesis in BAT, suggesting the role of different Htr in WAT. Indeed, in vitro experiments using 3T3-L1 adipocytes showed that Htr2a agonist treatment increased lipid accumulation and 5-HT suppressed lipolysis. Taken together, these results suggested that 5-HT could regulate energy storage in WAT through Htr2a and energy expenditure in BAT through Htr3.

In the present study, we provide evidence for a complex model, explaining the regulation of energy metabolism in different adipose tissues (Fig. 10f). In the over-fed state, 5-HT level increased in WAT, leading to the augmentation of lipogenesis via Htr2a. 5-HT also suppressed thermogenesis in the BAT via Htr3. When 5-HT signalling was inhibited, lipogenesis decreased in the eWAT and thermogenesis increased in both iWAT and BAT. The β3AR signalling stimulated by an HFD coupled with uninhibited thermogenesis by the blocking of Htr3 signalling resulted in enhanced energy expenditure in BAT. Thus, the inhibition of 5-HT production in adipose tissues may represent a novel strategy for anti-obesity treatment.

Methods

Reagents

PCPA, D-glucose, insulin, CL 316243, ondansetron, m-CPBG, triiodothyronine (T3), 3-isobutyl-1-methylxanthine (IBMX), indomethacin, dexamethasone, Oil Red O dye, ketanserin, DOI, isopropyl alcohol (IPA), formalin, ascorbic acid, perchloric acid, tamoxifen and polyethylene glycol 400 (PEG-400) were purchased from Sigma (St Louis, MO, USA). LP-533401 was purchased from Dalton Pharma Services (Toronto, Ontario, Canada). TRIzol reagent, DMEM medium, calf serum, fetal bovine serum (FBS) and penicillin/streptomycin (P/S) were obtained from Invitrogen (Carlsbad, CA, USA).

Animals and diets

The generation of Tph1flox/flox mice, Adipoq-Cre mice and aP2-CreERT2 mice has previously been reported37,38,39. C57BL/6 J mice, Htr3a KO mice (B6.129X1-Htr3atm1jul/J) and ob/ob mice (B6.V-Lepob/J) were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). To generate the adipocyte-specific Tph1 KO (Tph1 FKO) mice and inducible Tph1 KO (Tph1 AFKO) mice, Tph1flox/flox mice were crossed with Adipoq-Cre mice and aP2-CreERT2 mice, respectively. The mice were housed in climate-controlled, specific pathogen-free barrier facilities under a 12-h light–dark cycle, and chow and water were provided ad libitum. The Institutional Animal Care and Use Committee at the Korea Advanced Institute of Science and Technology approved the experimental protocols for this study. Htr3a KO mice and Tph1flox/flox mice were backcrossed with C57BL/6 J mice for more than ten generations. Cre recombination of 6-week-old Tph1 AFKO mice was induced by intraperitoneal injection of five doses of 2 mg of tamoxifen (Sigma) for a week. Male mice (aged 8∼10 weeks) were fed either an SCD (12% fat calories, Purina Laboratory Rodent Diets 38057) or an HFD (60% fat calories, Research Diets D12492). PBS or 300 mg kg−1 PCPA was administered as a daily intraperitoneal injection. LP-533401 was dissolved in PEG-400 and 5% dextrose (40:60 ratio). Vehicle or 30 mg kg−1 LP-533401 was administered daily with a feeding needle. We randomly divided C57BL/6J mice into two to approximately four groups. For transgenic mice, we compared data between KO mice and their WT littermates. No blinding was performed.

Cell culture

Murine 3T3-L1 cells (American Type Culture Collection) were cultured in DMEM supplemented with 10% FCS and 100 μg ml−1 P/S in a humidified atmosphere of 5% CO2 at 37 °C. Two days after reaching confluence, the cells were induced to differentiate using medium supplemented with 0.5 mM IBMX, 1 mg ml−1 insulin and 1 μM dexamethasone (day 0). After 2 days, the medium was replaced with DMEM supplemented with 1 mg ml−1 insulin in 10% FBS and P/S (day 2), and it was changed every 2 days from day 4 to 8. IBA were cultured in DMEM supplemented with 10% FBS and P/S in a humidified atmosphere of 5% CO2 at 37 °C40. After reaching 95% confluence, the cells were induced to differentiate using DMEM with 10% FBS, 0.5 μg ml−1 insulin, 1 nM T3, 0.125 mM indomethacin, 2 μg ml−1 dexamethasone, 0.5 μM IBMX and P/S (day 0). After 2 days, the medium was replaced with DMEM supplemented with 10% FBS, 0.5 μg ml−1 insulin, 1 nM T3 and P/S (day 2), and it was changed every 2 days from day 4 to 8. Primary brown pre-adipocytes were prepared from newborn Htr3a KO mice and their WT littermates as described previously41. The interscapular BAT was isolated from mice at postnatal day 2∼3, minced and then incubated in isolation buffer (0.123 M NaCl, 5 mM KCl, 1.3 mM CaCl2, 5 mM glucose, 100 mM HEPES and 4% BSA) containing 1 mg ml−1 collagenase type II (LS004176, Worthington) in 37 °C shaking incubator for 45∼50 min. The digested tissue was filtered through a 100-μm mesh filter (BD Bioscience). These filtered cells were washed twice with culture media and these pre-adipocytes were then cultured in DMEM supplemented with 20% FBS and 100 μg ml−1 P/S in a humidified atmosphere of 5% CO2 at 37 °C. After reaching 95% confluence, the cells were induced to differentiate using DMEM with 20% FBS, 20 nM insulin, 1 nM T3, 0.125 mM indomethacin, 0.5 mM dexamethasone, 0.5 mM IBMX and P/S (day 0). After 2 days, these cells were maintained in DMEM supplemented with 20% FBS, 20 nM insulin, 1 nM T3 and P/S for 4–5 days until exhibiting a massive accumulation of fat droplets41. We confirmed that these cell lines were free from Mycoplasma infection.

SVF isolation

The SVF of BAT from 7-week-old mice was separated by collagenase digestion. Briefly, the adipose tissues were dissected, minced and digested with 0.2% collagenase A (Roche) in Hank's balanced salt solution (Sigma) for 45 min at 37 °C, with constant shaking. Mature adipocytes and connective tissues were separated from the cell pellet by centrifugation at 800g for 10 min at 4 °C. The cell pellet was then suspended with RBC lysis buffer (Sigma) and filtered through a 40-μm mesh filter (BD Bioscience). The pelleted stromal vascular cells were re-suspended in DMEM containing 10% FBS and seeded in six-well plates for adipogenic differentiation.

Oil Red O staining

After full differentiation (day 8), 3T3-L1 adipocytes were fixed with 3.7% (w/v) formaldehyde in PBS for 15 min at room temperature and then washed three times with PBS. The cells were then stained with filtered Oil Red O solution (1.5 mg ml−1 60% (v/v) IPA) for 30 min and rinsed twice with distilled water. To quantify the amount of Oil Red O staining, the cells were eluted with 100% IPA for 10 min and the absorbance densities of the extracts were measured at 520 nm, using a VersaMax microplate reader (Molecular Devices, Sunnyvale, CA, USA).

Metabolic analysis

To measure the metabolic rate, the mice were housed individually in an eight-chamber, open-circuit Oxymax/CLAMS (Columbus Instruments Comprehensive Lab Animal Monitoring System) system as previously described42. Each mouse was assessed for 72 h in the fed state to measure metabolic rates. The respiratory exchange ratio (RER=VCO2/VO2) and heat production (HP=(3.185+1.232 × RER) × VO2) were calculated43. PET imaging was performed using a microPET R4 scanner (Concorde Microsystems, Siemens) as previously described44.

Glucose tolerance test and insulin tolerance test

For the glucose tolerance tests, the mice were administered 2 g kg−1 D-glucose in PBS after overnight fasting. For the insulin tolerance tests, the mice were intraperitoneally injected with insulin (0.75 U kg−1) after 4 h fasting. The blood samples were obtained from tail veins at 0, 15, 30, 45, 60, 90 and 120 min after injection and glucose concentrations were measured using a Gluco DR Plus glucometer (Allmedicus, Korea).

Blood chemistry analysis

Tissue 5-HT was extracted by homogenization in extraction buffer containing 0.02% ascorbic acid in 0.1 M perchloric acid followed by centrifugation. The 5-HT levels in the supernatants were measured via the liquid chromatography–mass spectrometry method.

cAMP assay

Differentiated IBA were treated with 1 μM ondansetron or 100 nM m-CPBG or 1 μM CL 316243 for 15 min. CL 316243 was used as a positive control. The cAMP competitive ELISA (Promega) was performed according to the manufacturer's instruction. Briefly, cAMP was extracted by adding 0.1 M HCl with 0.5% Triton X-100 to the cells. After centrifugation at 600g for 10 min, the supernatant was used for the determination of cAMP levels by competitive cAMP ELISA.

Oxygen consumption rate (OCR) assay

The OCR of the cells was measured using a Seahorse XF analyser (Seahorse Bioscience, Billerica, MA, USA). After seeding IBA on an XF-24 plate, the cells were incubated and differentiated using the protocol described above. Fully differentiated IBA were pre-treated with PBS and ondansetron for 30 min. The IBA were then treated with the β3AR agonist and the mitochondrial inhibitors oligomycin and rotenone/antimycin. The OCRs were calculated and recorded by a sensor cartridge and the Seahorse XF-24 software.

Quantitative reverse transcriptase-PCR (qRT-PCR) analysis

Total RNA was extracted from the mouse tissues or cell lines using TRIzol reagent, according to the manufacturer's protocol. After TURBO DNase (Invitrogen) treatment, 2 μg of total RNA was used to generate complementary DNA with Superscript III reverse transcriptase (Invitrogen). To analyse gene expression, real-time PCR was performed with a ViiA 7 Real-Time PCR system (Applied Biosystems) and the Power SYBR Green PCR master mix (Applied Biosystems). Relative quantification was based on the ddCt method and ActB was used as an endogenous control (internal control). The primer sequences are provided in the Supplementary Table 1.

Histological analysis

Inguinal, epididymal and interscapular adipose tissues were harvested, fixed in 4% (w/v) paraformaldehyde in PBS and embedded in paraffin. Then, 5-μm-thick tissue sections were deparaffinized, rehydrated and used for haematoxylin and eosin staining, immunohistochemistry and immunofluorescence. For antigen retrieval, the slides were submerged in 10 mM sodium citrate (pH 6.0) and heated to 95 °C for 20 min. Visualization of Ucp1 and Plin1 was performed using a VECTASTAIN ABC Kit (PK-4001, Vector Laboratories, Burlingame, CA, USA), according to the manufacturer's instructions. Briefly, the slides were incubated with BLOXALL Blocking solution (SP-6000, Vector Laboratories) followed by incubation with 2% normal goat serum for 30 min at room temperature, to block nonspecific binding. Sections were incubated with primary antibody against Ucp1 (ab10983, Abcam) or Plin1 (ab3526, Abcam) for 1 h at room temperature, followed by 30 min incubation with a species-specific, biotinylated secondary antibody. The slides were incubated with Vectastain ABC-AP reagent for 30 min and then incubated with alkaline phosphatase substrate (DAB, SK-4100, Vector Laboratories) for visualization. The stains and antibodies used for the immunofluorescence staining included BODIPY (BODIPY493/503, Invitrogen), anti-5-HT (ab10385, Abcam) and DAPI (D9542, Sigma). Electron microscopy images of BAT were obtained by transmission electron microscopy (Tecnai Spirit TEM) as previously described45. Briefly, the BAT was first fixed with 2.5% glutaraldehyde, and after fixation ultra-thin sections were cut, stained with uranyl acetate and lead citrate, and then examined under an electron microscope.

Western blot analysis

Whole-cell lysates were extracted by incubating cells in RIPA buffer (25 mM Tris-HCl pH 7.6, 150 mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS) plus protease inhibitors (Roche). Supernatants were collected following a brief centrifugation and protein concentrations in the supernatants were measured using the BCA Protein Assay Kit (Thermo Scientific, Rockford, IL, USA). The cell lysates were then mixed with equal volumes of 2 × Laemmli buffer (4% SDS, 20% glycerol, 10% 2-mercaptoethanol, 0.01% bromophenol blue and 120 mM Tris-HCl pH 6.8) and boiled for 5 min at 95 °C. Next, the protein samples were separated by SDS–PAGE and transferred to a polyvinylidene difluoride membrane (Millipore). After blocking in a 5% skim milk solution (Sigma), the membranes were incubated with the following specific primary antibodies: anti-phospho-HSL (Ser660) antibody (diluted 1:500, Cell Signaling #4126), anti-phospho-(Ser/Thr) protein kinase A substrate antibody (diluted 1:500, Cell Signaling #9621) and anti-Act antibody (diluted 1:1000, Cell Signaling #3700). The membranes were then washed with 1 × TBST and incubated with anti-rabbit IgG horseradish peroxidase-linked antibody or anti-mouse IgG antibody. The detection of each protein was performed using Supersignal West Pico Chemiluminescent Substrate (Thermo Scientific), according to the manufacturer's instructions. Signals were captured by a ChemiDoc MP system (Bio-Rad).

Glycerol release assay

Lipolysis was measured as the rate of glycerol release using a free glycerol reagent (Sigma), following the manufacturer's protocol. Briefly, fully differentiated 3T3-L1 adipocytes in a 24-well plate were incubated with 5-HT or DOI in Krebs Ringer phosphate buffer (136 mM NaCl, 4.7 mM KCl, 10 mM NaPO4, 0.9 mM MgSO4 and 0.9 mM CaCl2) containing 4% fatty acid-free BSA (Sigma) for 24 h. Isoproterenol was used as a positive control. After incubation, 10 μl of the cell culture supernatant was mixed with 0.8 ml of the free glycerol reagent and the mixture was then incubated at 37 °C for 5 min. The absorbance of the sample was determined at 540 nm using a spectrophotometer (DU730 Life Science UV/Vis, Beckman Coulter, Indianapolis, IN, USA). The amount of released glycerol was expressed relative to the cellular protein content.

Statistics

All values are expressed as the mean and s.e.m. The groups were compared by Student's t-test or one-way analysis of variance (ANOVA) or two-way ANOVA. Normal distribution was tested by the f-test. P-values <0.05 were considered statistically significant.

Author contributions

C.-M.O., J.N., I.K.L., S.P. and H.K. generated the hypothesis, designed the experiments and wrote the manuscript. M.S., V.Y., G.K., S.K., C.-M.O., J.N., Y.G., K.E.S., K.K., H.K., B.Y.B., H.W.L., Y.H.J., J.H.S., I.K.L., S.P. and H.K. performed experiments and analysed data.

Additional information

How to cite this article: Oh, C.-M. et al. Regulation of systemic energy homeostasis by serotonin in adipose tissues. Nat. Commun. 6:6794 doi: 10.1038/ncomms7794 (2015).

Supplementary Material

Supplementary Information

Supplementary Figures 1-4 and Supplementary Table 1

ncomms7794-s1.pdf (325.4KB, pdf)

Acknowledgments

We thank Hee-Saeng Jung and Taechang Yang for their technical support and Sang-Hee Lee for conducting the TEM imaging. We also thank Drs Chul-Ho Lee, Hak Chul Jang, Sung Hee Choi, Evan Rosen and Daniel Metzger, as well as the members of MDRL, for their helpful discussions and for sharing the transgenic mice. This work was supported by grants from the National Research Foundation (NRF) funded by the Ministry of Science, ICT & Future Planning (grant numbers: NRF-2012M3A9B2027974 and NRF-2014M3A9D8034464 to H.K., NRF-2012R1A1A4A01014488 to S.P., NRF-2012R1A2A1A03002833 to M.S., NRF-2012R1A2A1A03670452 to I.K.L. and 2013H1A8A1003985 to H.K.) and grants from the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI) funded by the Ministry of Health & Welfare, Republic of Korea (grant numbers: HI11C1964 to H.K. and A111345 to I.K.L.).

References

  1. Zhang X., Beaulieu J. M., Sotnikova T. D., Gainetdinov R. R. & Caron M. G. Tryptophan hydroxylase-2 controls brain serotonin synthesis. Science 305, 217 (2004) . [DOI] [PubMed] [Google Scholar]
  2. Gershon M. D. & Tack J. The serotonin signaling system: from basic understanding to drug development for functional GI disorders. Gastroenterology 132, 397–414 (2007) . [DOI] [PubMed] [Google Scholar]
  3. Wade P. R. et al. Localization and function of a 5-HT transporter in crypt epithelia of the gastrointestinal tract. J. Neurosci. 16, 2352–2364 (1996) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gershon M. D. & Ross L. L. Location of sites of 5-hydroxytryptamine storage and metabolism by radioautography. J. Physiol. 186, 477–492 (1966) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Tecott L. H. Serotonin and the orchestration of energy balance. Cell. Metab. 6, 352–361 (2007) . [DOI] [PubMed] [Google Scholar]
  6. Berglund E. D. et al. Serotonin 2C receptors in pro-opiomelanocortin neurons regulate energy and glucose homeostasis. J. Clin. Invest. 123, 5061 (2013) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nonogaki K., Strack A. M., Dallman M. F. & Tecott L. H. Leptin-independent hyperphagia and type 2 diabetes in mice with a mutated serotonin 5-HT2C receptor gene. Nat. Med. 4, 1152–1156 (1998) . [DOI] [PubMed] [Google Scholar]
  8. Sohn J. W. et al. Serotonin 2C receptor activates a distinct population of arcuate pro-opiomelanocortin neurons via TRPC channels. Neuron 71, 488–497 (2011) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Breisch S. T., Zemlan F. P. & Hoebel B. G. Hyperphagia and obesity following serotonin depletion by intraventricular p-chlorophenylalanine. Science 192, 382–385 (1976) . [DOI] [PubMed] [Google Scholar]
  10. Savelieva K. V. et al. Genetic disruption of both tryptophan hydroxylase genes dramatically reduces serotonin and affects behavior in models sensitive to antidepressants. PLoS ONE 3, e3301 (2008) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Murphy D. L. & Lesch K. P. Targeting the murine serotonin transporter: insights into human neurobiology. Nat. Rev. Neurosci. 9, 85–96 (2008) . [DOI] [PubMed] [Google Scholar]
  12. Serretti A. & Mandelli L. Antidepressants and body weight: a comprehensive review and meta-analysis. J. Clin. Psychiatry 71, 1259–1272 (2010) . [DOI] [PubMed] [Google Scholar]
  13. Koe B. K. & Weissman A. p-Chlorophenylalanine: a specific depletor of brain serotonin. J. Pharmacol. Exp. Ther. 154, 499–516 (1966) . [PubMed] [Google Scholar]
  14. Liu Q. et al. Discovery and characterization of novel tryptophan hydroxylase inhibitors that selectively inhibit serotonin synthesis in the gastrointestinal tract. J. Pharmacol. Exp. Ther. 325, 47–55 (2008) . [DOI] [PubMed] [Google Scholar]
  15. Uchida-Kitajima S. et al. 5-Hydroxytryptamine 2A receptor signaling cascade modulates adiponectin and plasminogen activator inhibitor 1 expression in adipose tissue. FEBS Lett. 582, 3037–3044 (2008) . [DOI] [PubMed] [Google Scholar]
  16. Mann J. J. et al. Relationship between central and peripheral serotonin indexes in depressed and suicidal psychiatric inpatients. Arch. Gen. Psychiatry 49, 442–446 (1992) . [DOI] [PubMed] [Google Scholar]
  17. Sumara G., Sumara O., Kim J. K. & Karsenty G. Gut-derived serotonin is a multifunctional determinant to fasting adaptation. Cell Metab. 16, 588–600 (2012) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kinoshita M. et al. Regulation of adipocyte differentiation by activation of serotonin (5-HT) receptors 5-HT2AR and 5-HT2CR and involvement of microRNA-448-mediated repression of KLF5. Mol. Endocrinol. 24, 1978–1987 (2010) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Stunes A. et al. Adipocytes express a functional system for serotonin synthesis, reuptake and receptor activation. Diabetes Obes. Metab. 13, 551–558 (2011) . [DOI] [PubMed] [Google Scholar]
  20. Nicholls D. G. & Locke R. M. Thermogenic mechanisms in brown fat. Physiol. Rev. 64, 1–64 (1984) . [DOI] [PubMed] [Google Scholar]
  21. Wu J. et al. Beige adipocytes are a distinct type of thermogenic fat cell in mouse and human. Cell 150, 366–376 (2012) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Seale P. et al. Prdm16 determines the thermogenic program of subcutaneous white adipose tissue in mice. J. Clin. Invest. 121, 96–105 (2011) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Maricq A. V., Peterson A. S., Brake A. J., Myers R. M. & Julius D. Primary structure and functional expression of the 5HT3 receptor, a serotonin-gated ion channel. Science 254, 432–437 (1991) . [DOI] [PubMed] [Google Scholar]
  24. Derkach V., Surprenant A. & North R. A. 5-HT3 receptors are membrane ion channels. Nature 339, 706–709 (1989) . [DOI] [PubMed] [Google Scholar]
  25. Ohara-Imaizumi M. et al. Serotonin regulates glucose-stimulated insulin secretion from pancreatic β cells during pregnancy. Proc. Natl Acad. Sci. 110, 19420–19425 (2013) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Kim K. et al. Functional role of serotonin in insulin secretion in a diet-induced insulin-resistant state. Endocrinology 156, 444–452 (2014) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Rosmond R. Association studies of genetic polymorphisms in central obesity: a critical review. Int. J. Obes. 27, 1141–1151 (2003) . [DOI] [PubMed] [Google Scholar]
  28. Chen C. et al. Genetic variations in the serotoninergic system contribute to body-mass index in Chinese adolescents. PLoS ONE 8, e58717 (2013) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Li P. et al. Genetic association analysis of 30 genes related to obesity in a European American population. Int. J. Obes. 38, 724–729 (2014) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Kim H.-J. et al. Metabolomic analysis of livers and serum from high-fat diet induced obese mice. J. Proteome Res. 10, 722–731 (2010) . [DOI] [PubMed] [Google Scholar]
  31. Haub S. et al. Serotonin receptor type 3 antagonists improve obesity-associated fatty liver disease in mice. J. Pharmacol. Exp. Ther. 339, 790–798 (2011) . [DOI] [PubMed] [Google Scholar]
  32. Levitan R. et al. The serotonin-1Dβ receptor gene and severity of obsessive-compulsive disorder in women with bulimia nervosa. Eur. Neuropsychopharmacol. 16, 1–6 (2006) . [DOI] [PubMed] [Google Scholar]
  33. Gorwood P. et al. The 5-HT2A-1438G/A polymorphism in anorexia nervosa: a combined analysis of 316 trios from six European centres. Mol. Psychiatry 7, 90–94 (2002) . [DOI] [PubMed] [Google Scholar]
  34. Compan V. et al. Serotonin signaling in eating disorders. Wiley Interdisciplinary Reviews: Membrane Transport and Signaling 1, 715–729 (2012) . [Google Scholar]
  35. Crane J. D. et al. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis. Nat. Med. 21, 166–172 (2014) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Girardier L. & Schneider-Picard G. Alpha and beta-adrenergic mediation of membrane potential changes and metabolism in rat brown adipose tissue. J. Physiol. 335, 629–641 (1983) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Yadav V. K. et al. Lrp5 controls bone formation by inhibiting serotonin synthesis in the duodenum. Cell 135, 825–837 (2008) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Eguchi J. et al. Transcriptional control of adipose lipid handling by IRF4. Cell Metab. 13, 249–259 (2011) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Imai T. et al. Impaired adipogenesis and lipolysis in the mouse upon selective ablation of the retinoid X receptor alpha mediated by a tamoxifen-inducible chimeric Cre recombinase (Cre-ERT2) in adipocytes. Proc. Natl Acad. Sci. USA 98, 224–288 (2001) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Uldry M. et al. Complementary action of the PGC-1 coactivators in mitochondrial biogenesis and brown fat differentiation. Cell Metab. 3, 333–341 (2006) . [DOI] [PubMed] [Google Scholar]
  41. Klein J. et al. β3-Adrenergic stimulation differentially inhibits insulin signaling and decreases insulin-induced glucose uptake in brown adipocytes. J. Biol. Chem. 274, 34795–34802 (1999) . [DOI] [PubMed] [Google Scholar]
  42. Bernal-Mizrachi C. et al. Respiratory uncoupling lowers blood pressure through a leptin-dependent mechanism in genetically obese mice. Arterioscler. Thromb. Vasc. Biol. 22, 961–968 (2002) . [DOI] [PubMed] [Google Scholar]
  43. McLean J. & Tobin G. Animal and Human Calorimetry Cambridge University Press (1987) . [Google Scholar]
  44. Wang X., Minze L. & Shi Z. Functional imaging of brown fat in mice with 18F-FDG micro-PET/CT. J. Vis. Exp. 69, e4060 (2011) . [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Ozaki K., Sano T., Tsuji N., Matsuura T. & Narama I. Carnitine is necessary to maintain the phenotype and function of brown adipose tissue. Lab. Invest. 91, 704–710 (2011) . [DOI] [PubMed] [Google Scholar]

Associated Data

This section collects any data citations, data availability statements, or supplementary materials included in this article.

Supplementary Materials

Supplementary Information

Supplementary Figures 1-4 and Supplementary Table 1

ncomms7794-s1.pdf (325.4KB, pdf)

Articles from Nature Communications are provided here courtesy of Nature Publishing Group

RESOURCES