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. 2015 Apr 13;6:6794. doi: 10.1038/ncomms7794

Figure 3. PCPA decreased lipogenesis in eWAT and induces brown fat-like changes in iWAT.

Figure 3

(a) Representative haematoxylin and eosin (H&E) images of eWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (b) Average adipocyte sizes of eWAT were measured from H&E images using ImageJ software. n=5 mice per group. *P<0.05 and versus vehicle by Student's t-test. ***P<0.001 versus SCD+vehicle and HFD+PCPA by two-way analysis of variance (ANOVA). (c) Expression of genes associated with lipogenesis in eWAT, as assessed by quantitative reverse transcriptase–PCR (qRT–PCR). n=4 mice per group. *P<0.05 and ***P<0.001 versus vehicle by Student's t-test. (d) Representative H&E images of iWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (e) Average adipocyte sizes of iWAT were measured from H&E images using ImageJ software. n=5 mice per group. ***P<0.001 versus SCD+vehicle and HFD+PCPA by two-way ANOVA. (f) Immunohistochemical staining for Ucp1 in iWAT from vehicle- or PCPA-treated mice after 8 weeks of HFD feeding. (g) Ucp1 gene expression in iWAT was assessed via qRT–PCR. n=4 mice per group. ***P<0.001 versus vehicle by Student's t-test. All data are presented as the mean±s.e. Scale bar, 20 μm.