Figure 4. LP-533401 protects against diet-induced obesity and increased BAT activity.
Mice were treated orally with vehicle or LP-533401 (30 mg kg−1) and fed an SCD or HFD for 14 weeks from 8 weeks of age. (a) Growth curves of vehicle- or LP-533401-treated mice fed an HFD. n=5 mice per group. *P<0.05, **P<0.01 and ***P<0.001 versus HFD+LP-533401 by Student's t-test. (b) Intraperitoneal glucose tolerance test (IPGTT) after 16 h fasting n=4 mice per group. *P<0.05 and **P<0.01 versus HFD+LP-533401 by Student's t-test. (c) Representative haematoxylin and eosin (H&E) images of BAT of vehicle- or LP-533401-treated mice. Scale bar, 20 μm. (d) Expression of thermogenesis-related genes in BAT was assessed by quantitative reverse transcriptase–PCR (qRT–PCR). n=4 mice per group. *P<0.05 versus vehicle by Student's t-test. All data are presented as the mean±s.e.