FIG 2.

BLI sensograms of binding events between Nullbasic and RT. The Octet Red96 system was used to measure binding events between Nullbasic and RT. (A and B) BLI sensograms. Biotinylated RT p51/66 was bound to a streptavidin biosensor and applied to solutions containing 10 nM (green), 30 nM (light blue), 90 nM (red), or 270 nM (dark blue) of Nullbasic-FLAG-V5-6×His (A) or (B) BSA. The BLI experiment was repeated at three times with similar results. The results of a representative sensogram are shown.