FIG 7.

Effect of Nullbasic on virion morphology. (A) Representative electron micrograph images illustrating mature, eccentric, and immature particle morphologies. (B) Frequencies of the different core morphologies for HIV-1 produced by HEK293T cells transfected with pGCH proviral plasmid and pcDNA3.1-FLAG (black bars) or pcDNA3.1-Nullbasic-FLAG (gray bars). Mean values and standard errors of the mean were determined by comparing three independent sets of 60 to 180 virus particles each. Statistical analysis was performed using a Student t test from at least three independent measurements. The statistical significance was set at P < 0.05. (C) Western blot of lysates from the transfected cells using anti-capsid, anti-FLAG, or anti-tubulin antibodies.