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. 2015 Feb 18;89(9):5097–5109. doi: 10.1128/JVI.03264-14

FIG 5.

FIG 5

The HCMV homologue of ORF57 also enhances K15 expression. (A) HEK293T cells were cotransfected with K15 cDNA and ORF57 or HCMV pUL69. Protein was harvested at 40 h posttransfection and analyzed by Western blotting. Western blot of cotransfections with K15c and either ORF57 or HCMV pUL69. Detection and quantification were carried out with an Odyssey infrared imaging system. The expression of K15 alone was set to 1. The quantification represents three independent experiments. (B) Activity test of UL69 with UL11 an AT-rich RNA from HCMV. HEK293T cells were cotransfected with HCMV UL11 and pUL69 or KSHV ORF57. Protein was harvested at 40 h posttransfection and analyzed by Western blotting. V5-tagged UL11 was detected by an anti-V5 antibody.