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TAg alone is not sufficient to fully activate ATR-mediated DDR. RPTE cells were either transduced with empty or TAg-expressing lentivirus or infected with BKPyV at the designated MOIs for 3 days. Total proteins from cells grown at the different temperatures were harvested at the indicated times postinfection and probed for the indicated proteins by Western blotting. The blots shown are representative of three independent experiments.
