FIG 5.

The replication-deficient TAg D501A mutant virus is unable to activate the DDR. (A) Alignment of SV40 and BKPyV TAg cartoon structures as described in the legend to Fig. 3A, with the Cβ and the Cγ atoms of the D501 residue of BKPyV TAg depicted as red spheres. (B) RPTE cells were infected with wild-type or TAg D501A mutant BKPyV at an MOI of 0.5 IU/cell. Low-molecular-weight DNA was harvested at 1 day p.i. and 3 days p.i., and BKPyV DNA was quantified by quantitative PCR in triplicates. Replicated DNA (3 days p.i.) was normalized to input DNA (1 day p.i.). Data shown are for one experiment that is representative of three independent experiments. The error bars show the SD values. ****, P < 0.0001. (C) Total proteins were harvested at 1 day p.i. and 3 days p.i. and probed for the indicated proteins by Western blotting. The blots shown are representative of at least three independent experiments.