FIG 7.

DNA damage accumulates in TAg D501A mutant virus-infected cells. An alkaline comet assay was used to determine host DNA damage. (A) Representative comet assay images of mock-infected cells (top) and cells infected with WT BKPyV (middle) and TAg D501A mutant virus (bottom). Scale bar, 100 μm. (B) Box-and-whisker plots demonstrating the distribution of DNA damage. Shown are combined results from three independent experiments. The top and bottom of the whiskers represent the maximum and minimum data points in the data set, respectively. The three horizontal lines of the boxes denote the first quartile, median, and third quartile, starting with the lowest line. The plus (+) sign in each plot is the average of the data. At least 50 events were scored per condition per experiment. **, P < 0.01.