FIG 4.

Effect of ganciclovir treatment on expression of TBPC regulatory proteins. (A) TBPCs were infected with VR1814 with ganciclovir (GCV) (+) or without GCV (−), fixed at 5 days p.i., and immunostained for viral proteins UL112/113 and pp28. Original magnification, ×200. Bar = 20 μm. (B) Immunoblot of TBPC regulatory proteins (geminin, HMGA2, and GATA4) in uninfected controls and infected cells with GCV. (C) Immunofluorescence analysis of GATA3 and Hand1 in uninfected controls and infected cells with ganciclovir. Cellular proteins (green), HCMV IE1 (red), and DAPI (blue) are indicated. Colocalization of red and green signals (yellow) and of red, green, and blue signals (white) are indicated. Original magnification, ×200. Bar = 20 μm.