TABLE 1.
Oligonucleotides used in this study
| Name | Orientationa | Sequence (5′ → 3′) |
|---|---|---|
| BNI55 | + | CATGCCATGGTCATGTCCCCTATACTAGGTTATTGG |
| BNI56 | − | TCCCCGCGGAATGCAGGGGCCCCTGGAACAGAAC |
| BNI57 | + | GTTCCGCGGGAGAAATACATGCTGTCCC |
| BNI59 | + | CATGGACTACAAGGACGACGACGACAAGGCCGC |
| BNI60 | − | GGCCTTGTCGTCGTCGTCCTTGTAGTC |
| BNI61 | − | CCGCTCGAGCTACCTCCTACCTTCAGCAAACTTAATA |
| BNI79 | − | CCGCTCGAGTTATCTCCTAGCTCCCCTGACATG |
| BNI99 | + | CATGCCATGGCAAGTGGGGATGTCTTGTGGGATATTC |
| BNI100 | + | TCCCCGCGGCAAGTATCCCAGTGAATGAGGCAC |
| BNI232 | − | GCTCTAGACTACCTTCGCCCAAATATGCGGGTTGC |
| BNI237 | + | CTGTGAGCTTCCTCGAGAGTGATGGGTGTTG |
| BNI239 | + | CTAAGGAAAAGACTCGAGCCAAAGCAAATG |
| BNI241 | + | GTGGGATTGCATCTCGAGGAGATGAACAATG |
| BNI243 | + | CCCTATGGAGCCTCGAGGAACGCCTTGTG |
| BNI245 | + | GCATCAAATACCCTCGAGCCCCTCATGGC |
| BNI247 | + | CTTCACCAGAATCTCGAGGACACCTCCATG |
| BNI249 | + | GCATATTTGGGCTCGAGAGTCTAGACTAC |
| BNI263 | − | CTAGTCTAGACTACTTCTGCATGGAGGTGTCCTTGA |
| BNI264 | − | CTAGTCTAGACTAAGGGCTCCATAGGGTCCTGAGC |
| BNI265 | − | CTAGTCTAGACTACTGTCTTTTCCTTAGGACCACTTCC |
| BNI277 | − | CTAGTCTAGACTACATGCTCACCAAACCAAAAGG |
| BNI335 | − | GAAGATCTAGCTGTAACCCAGGAGCGCAC |
| BNI339 | + | CGGGATCCGCGGTGGCATGGCTTCCAAGGTGTACGACCCCG |
| BNI340 | − | GCTCTAGACTGCTCGTTCTTCAGCACTCGCTC |
| BNI343 | + | GCTCTAGAAACTTTGATTTATTAAAATTAGCAGGAGATGTCGAAT |
| BNI382 | + | TCGAGTACCCGTACGACGTCCCGGACTACGCGC |
| BNI383 | − | TCGAGCGCGTAGTCCGGGACGTCGTACGGGTAC |
| BNI396 | + | CTGTTGACACCTCTCGAGGTCACTATGGCTG |
| BNI470 | − | CGGGATCCCAAGATGGTATTTGATGCTTTC |
| Bo89 | + | TGGACGCAGTCTTTGAATACAACC |
| Bo370 | − | ACCTGATCCGTGGATCTCACCAGCTGTAACCCAGGAGCGCACCAGA |
| Bo371 | + | GGTAGCGGAGGAGAAATACATGCTGTCCCTTTTG |
| Bo372 | + | GCTGGTGAGATCCACGGATCAGGTTACCCATACGATGTCCCAGACTACGCT |
| Bo373 | − | ATGTATTTCTCCTCCGCTACCAGCGTAGTCTGGGACATCGTAACCG |
| Bo508 | − | CTAGTCTAGACTATTTCCTTAGGACCACTTCCATTGC |
| Bo510 | − | CTAGTCTAGACTATAGGACCACTTCCATTGCTATCAT |
| Bo546 | − | CTAGTCTAGACTACATTGCTATCATCATGCTCACCAA |
| Bo547 | − | CTAGTCTAGACTATATCATCATGCTCACCAAACC |
| Bo698 | − | CTAGTCTAGACTACACTTCCATTGCTATCATCATGCTC |
| RU2501 | − | GGAATTCTACCTTCGCCCAAATATGCGGGT |
a
+, forward orientation; −, reverse orientation. For primers used in site-directed mutagenesis, only forward primers are shown; the corresponding complementary reverse primers were also used.