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. 2015 Feb 18;89(9):5164–5170. doi: 10.1128/JVI.02351-14

TABLE 1.

List of oligonucleotides used for cloning and real-time quantitative PCRa

Primer (assay) Sequence
core 630 JFH1 5′-GGCGTCTTCCCCAGATCTCCATCCTGCCCAGCC-3′
core+1 630 JFH1 5′-GGCGTCTTCCCCAGATCTGCCATCCTGCCCAGCC-3′
core-1 630 JFH1 5′-GGCGTCTTCCCCAGATCTTCACCTGCCCAGCC-3′
core+1 630 H77 5′-GGCGTCTTCCCCAGATCTGCCATCCCGCCCACCC-3′
N328b 5′-CTGTCTTCACGCAGAAAGCG-3′
N329c 5′-CCGAACGGACATTTCGAAG-3′
N323Bd 5′-GGGGAAGACGCCAAAAAC-3′
CD20 M F 5′-CCCAGAAGACATTAAGTTCCC-3′
CD20 M R 5′-GGGAACTTAATGTCTTCTGGG-3′
CD21S F 5′-CCAGAAGACGTGAAGTTCCCG-3′
CD21S R 5′-CGGGAACTTCACGTCTTCTGG-3′
CD25/26 M F 5′-AGTTCCCGGGGGGGGGCCAGATCG −3′
CD25/26 M R 5′-CGATCTGGCCCCCCCCCGGGAACT −3′
CD14S F 5′-CCAAAAGAAACATGAACCCGTCGCCCAGAAG-3′
CD14S R 5′-CTTCTGGGCGACGGTTCATGTTTCTTTTGG-3′
CD28S F 5′-CGGCGGCCTGATCGTTGGCGGAG-3′
CD28S R 5′-GCCAACGATCAGGCCGCCGCCCG-3′
CD48S F 5′-GGTGTGCGCACGATGAGGAAAACTACGG-3′
CD48S R 5′-CCGAAGTTTTCCTCATCGTGCGCACACC-3′
CD85/87S F 5′-GGCCCCTATGGGGGAGGGAGGGACTCG-3′
CD85/87S R 5′-CGAGTCCCTCCCTCCCCCATAGGGGCC-3′
CD87S F 5′-CTATATGGGATAGAGGGACTCGGC-3′
CD87S R 5′-AGCCGAGTCCCTCTATCCCATATAG-3′
CD90S F 5′-GGAATGAGGGACTAGGCTGGGCAGG-3′
CD90S R 5′-CCTGCCCAGCCTAGTCCCTCATTCC-3′
JFH1-5NTR276F (RT-PCR for IRES) 5′-GGCCTTGTGGTACTGCCTGATA-3′
JFH1-5NTR354R (RT-PCR for IRES) 5′-GGATTTGTGCTCATGGTGCA-3′
YWHAZR 5′-GGATGTGTTGGTTGCATTTCCT-3′
YWHAZF 5′-GCTGGTGATGACAAGAAAGG-3′
a

Mutated codons are underlined.

b

Forward for core 630 JFH1, core+1 630 JFH1, core-1 630 JFH1, core+1 630 H77, and all reverse primers (R) of STable2.

c

Reverse for N323B and all forward primers (F) of STable2.

d

Forward for N329 in AUG→GGG of luciferase original start.