TABLE 1.
Primer (assay) | Sequence |
---|---|
core 630 JFH1 | 5′-GGCGTCTTCCCCAGATCTCCATCCTGCCCAGCC-3′ |
core+1 630 JFH1 | 5′-GGCGTCTTCCCCAGATCTGCCATCCTGCCCAGCC-3′ |
core-1 630 JFH1 | 5′-GGCGTCTTCCCCAGATCTTCACCTGCCCAGCC-3′ |
core+1 630 H77 | 5′-GGCGTCTTCCCCAGATCTGCCATCCCGCCCACCC-3′ |
N328b | 5′-CTGTCTTCACGCAGAAAGCG-3′ |
N329c | 5′-CCGAACGGACATTTCGAAG-3′ |
N323Bd | 5′-GGGGAAGACGCCAAAAAC-3′ |
CD20 M F | 5′-CCCAGAAGACATTAAGTTCCC-3′ |
CD20 M R | 5′-GGGAACTTAATGTCTTCTGGG-3′ |
CD21S F | 5′-CCAGAAGACGTGAAGTTCCCG-3′ |
CD21S R | 5′-CGGGAACTTCACGTCTTCTGG-3′ |
CD25/26 M F | 5′-AGTTCCCGGGGGGGGGCCAGATCG −3′ |
CD25/26 M R | 5′-CGATCTGGCCCCCCCCCGGGAACT −3′ |
CD14S F | 5′-CCAAAAGAAACATGAACCCGTCGCCCAGAAG-3′ |
CD14S R | 5′-CTTCTGGGCGACGGTTCATGTTTCTTTTGG-3′ |
CD28S F | 5′-CGGCGGCCTGATCGTTGGCGGAG-3′ |
CD28S R | 5′-GCCAACGATCAGGCCGCCGCCCG-3′ |
CD48S F | 5′-GGTGTGCGCACGATGAGGAAAACTACGG-3′ |
CD48S R | 5′-CCGAAGTTTTCCTCATCGTGCGCACACC-3′ |
CD85/87S F | 5′-GGCCCCTATGGGGGAGGGAGGGACTCG-3′ |
CD85/87S R | 5′-CGAGTCCCTCCCTCCCCCATAGGGGCC-3′ |
CD87S F | 5′-CTATATGGGATAGAGGGACTCGGC-3′ |
CD87S R | 5′-AGCCGAGTCCCTCTATCCCATATAG-3′ |
CD90S F | 5′-GGAATGAGGGACTAGGCTGGGCAGG-3′ |
CD90S R | 5′-CCTGCCCAGCCTAGTCCCTCATTCC-3′ |
JFH1-5NTR276F (RT-PCR for IRES) | 5′-GGCCTTGTGGTACTGCCTGATA-3′ |
JFH1-5NTR354R (RT-PCR for IRES) | 5′-GGATTTGTGCTCATGGTGCA-3′ |
YWHAZR | 5′-GGATGTGTTGGTTGCATTTCCT-3′ |
YWHAZF | 5′-GCTGGTGATGACAAGAAAGG-3′ |
Mutated codons are underlined.
Forward for core 630 JFH1, core+1 630 JFH1, core-1 630 JFH1, core+1 630 H77, and all reverse primers (R) of STable2.
Reverse for N323B and all forward primers (F) of STable2.
Forward for N329 in AUG→GGG of luciferase original start.