TABLE 1.
Primers and probes used for qRT-PCR
| Targeta | Sequenceb | Reference |
|---|---|---|
| OROV | 83 | |
| Fwd | 5′-TACCCAGATGCGATCACCAA-3′ | |
| Rev | 5′-TTGCGTCACCATCATTCCAA-3′ | |
| Probe | 5′-56-FAM-TGCCTTTGGCTGAGGTAAAGGGCTG-36-TAMSp-3′ | |
| Mouse Gapdh | 84 | |
| Fwd | 5′-AATGGTGAAGGTCGGTGTG-3′ | |
| Rev | 5′-GTGGAGTCATACTGGAACATGTAG-3′ | |
| Probe | 5′-56-FAM-TGCAAATGG-ZEN-CAGCCCTGGTG-3IABkFQ-3′ | |
| Mouse Ifna | 84 | |
| Fwd | 5′-CTTCCACAGGATCACTGTGTACCT-3′ | |
| Rev | 5′-TTCTGCTCTGACCACCTCCC-3′ | |
| Probe | 5′-56-FAM-AGAGAGAAGAAACACAGCCCCTGTGCC-36-TAMSp-3′ | |
| Mouse Ifnb | 52 | |
| Fwd | 5′-GGCTTCCATCATGAACAACAG-3′ | |
| Rev | 5′-GTTGATGGAGAGGGCTGTG-3′ | |
| Probe | 5′-56-FAM-CTGCGTTCCTGCTGTGCTTCTC-36-TAMSp-3′ | |
| Mouse Ifit1 | 52 | |
| Fwd | 5′-GAGCCAGAAAACCCTGAGTACA-3′ | |
| Rev | 5′-AGAAATAAAGTTGTCATCTAAATC-3′ | |
| Probe | 5′-56-FAM-ACTGGCTATGCAGTCGTAGCCTATCGCC-36-TAMSp-3′ |
a
Fwd, forward; Rev, reverse.
b
FAM, 6-carboxyfluorescein; TAMsp, 6-carboxytetramethylrhodamine; ZEN, double-quenched probe; 3IABkFQ, 3′ Iowa Black FQ.