. 2015 Feb 25;89(9):4720–4737. doi: 10.1128/JVI.00077-15

TABLE 2.

Serum cytokine and chemokine levels after OROV infection^a

Cytokine	Genotype	Day 4 p.i.		Day 6 p.i.
Cytokine	Genotype	pg/ml (±SD)	P	pg/ml (±SD)	P
IL-1α	WT	1.3 (±1.6)		2.6 (±2.3)
	Ifnar^−/−	5.3 (±8.3)	0.93
	Irf3^−/− × Irf7^−/−	10.6 (±11.5)	0.08	0.6 (±1.4)	0.05
	Mavs^−/−	0 (±0)	0.03	5.4 (±7.8)	0.96
IL-1β	WT	189.4 (±44.2)		208.2 (±53.4)
	Ifnar^−/−	266.0 (±179.8)	0.54
	Irf3^−/− × Irf7^−/−	205.7 (±135.5)	0.99	109.1 (±101.3)	0.04
	Mavs^−/−	123.5 (±95.1)	0.07	88.9 (±101.5)	0.02
IL-2	WT	25.0 (±14.6)		26.9 (±21.9)
	Ifnar^−/−	18.9 (±11.3)	0.55
	Irf3^−/− × Irf7^−/−	20.9 (±14.1)	0.51	14.9 (±11.9)	0.50
	Mavs^−/−	16.6 (±12.8)	0.05	28.3 (±12.7)	0.22
IL-3	WT	12.8 (±11.9)		16.5 (±13.3)
	Ifnar^−/−	5.13 (±7.19)	0.15
	Irf3^−/− × Irf7^−/−	8.6 (±9.9)	0.68	4.6 (±7.1)	0.09
	Mavs^−/−	3.6 (±3.3)	0.17	2.0 (±3.1)	0.008
IL-4	WT	6.9 (±8.4)		8.6 (±9.1)
	Ifnar^−/−	2.5 (±3.9)	0.30
	Irf3^−/− × Irf7^−/−	3.0 (±5.7)	0.20	2.1 (±5.0)	0.32
	Mavs^−/−	0 (±0)	0.06	0 (±0)	0.03
IL-5	WT	14.3 (±7.5)		16.2 (±7.1)
	Ifnar^−/−	10.2 (±3.1)	0.15
	Irf3^−/− × Irf7^−/−	6.2 (±4.2)	0.004	9.2 (±10.2)	0.17
	Mavs^−/−	8.8 (±5.8)	0.15	3.5 (±5.8)	0.002
IL-6	WT	4.2 (±1.5)		5.9 (±2.9)
	Ifnar^−/−	1,048 (±1,921)	0.00008
	Irf3^−/− × Irf7^−/−	606 (±1,055)	<0.0001	56.8 (±107.4)	0.76
	Mavs^−/−	5.8 (±4.8)	0.64	146.1 (±279.1)	0.0002
IL-9	WT	108.6 (±115.4)		116.5 (±131.9)
	Ifnar^−/−	337.9 (±410.3)	0.36
	Irf3^−/− × Irf7^−/−	159.7 (±282.9)	0.68	0 (±0)	0.03
	Mavs^−/−	96.3 (±167.6)	0.64	325.6 (±425.8)	0.45
IL-10	WT	22.8 (±16.3)		25.6 (±14.1)
	Ifnar^−/−	23.8 (±14.6)	0.75
	Irf3^−/− × Irf7^−/−	31.6 (±35.1)	0.63	10.9 (±6.9)	0.01
	Mavs^−/−	17.4 (±17.0)	0.31	16.7 (±15.0)	0.18
IL-12 (p40)	WT	42.8 (±30.9)		35.4 (±17.6)
	Ifnar^−/−	341.7 (±164.1)	<0.0001
	Irf3^−/− × Irf7^−/−	338.7 (±248.8)	<0.0001	32.6 (±25.1)	0.58
	Mavs^−/−	86.5 (±79.2)	0.09	69.7 (±77.6)	0.35
IL-12 (p70)	WT	18.6 (±12.2)		21.3 (±8.7)
	Ifnar^−/−	74.5 (±93.6)	0.51
	Irf3^−/− × Irf7^−/−	33.8 (±31.2)	0.81	13.3 (±11.9)	0.11
	Mavs^−/−	14.6 (±11.6)	0.55	21.8 (±29.0)	0.17
IL-13	WT	31.6 (±26.3)		47.6 (±52.2)
	Ifnar^−/−	30.3 (±31.9)	0.97
	Irf3^−/− × Irf7^−/−	47.2 (±61.9)	0.72	10.19 (±15.0)	0.04
	Mavs^−/−	14.0 (±25.4)	0.052	12.3 (±13.3)	0.03
IL-17	WT	7.7 (±5.1)		8.7 (±6.6)
	Ifnar^−/−	5.3 (±5.9)	0.15
	Irf3^−/− × Irf7^−/−	5.0 (±4.7)	0.12	5.1 (±4.3)	0.28
	Mavs^−/−	2.5 (±2.1)	0.02	2.4 (±1.6)	0.07
Eotaxin	WT	422.5 (±287.0)		478.5 (±230.7)
	Ifnar^−/−	451.5 (±462.0)	0.84
	Irf3^−/− × Irf7^−/−	344.2 (±292.9)	0.42	178.5 (±170.8)	0.005
	Mavs^−/−	145.1 (±189.2)	0.03	180.2 (±206.0)	0.01
G-CSF	WT	15.8 (±5.2)		13.6 (±5.5)
	Ifnar^−/−	3,064 (±4,307)	0.0004
	Irf3^−/− × Irf7^−/−	3,000 (±3,767)	<0.0001	106.7 (±146.5)	0.09
	Mavs^−/−	10.8 (±8.2)	0.15	1,307 (±2,543)	0.03
GM-CSF	WT	70.8 (±26.6)		74.8 (±29.3)
	Ifnar^−/−	86.5 (±32.8)	0.12
	Irf3^−/− × Irf7^−/−	63.5 (±23.9)	0.43	57.6 (±39.7)	0.23
	Mavs^−/−	43.9 (±19.1)	0.06	22.0 (±28.3)	0.03
IFN-γ	WT	2.5 (±2.1)		2.8 (±1.4)
	Ifnar^−/−	34.2 (±22.8)	0.0004
	Irf3^−/− × Irf7^−/−	4.9 (±3.9)	0.11	3.9 (±3.7)	0.94
	Mavs^−/−	1.5 (±1.5)	0.29	6.4 (±5.1)	0.10
KC	WT	28.7 (±9.5)		35.3 (±18.7)
	Ifnar^−/−	384.2 (212.1)	0.0008
	Irf3^−/− × Irf7^−/−	285.5 (196.0)	<0.0001	28.5 (±24.6)	0.23
	Mavs^−/−	61.6 (±59.9)	0.79	87.6 (±48.8)	0.008
MCP-1	WT	156.1 (±113.0)		202.0 (±132.8)
	Ifnar^−/−	716.6 (±267.5)	0.0003
	Irf3^−/− × Irf7^−/−	902.8 (±786.5)	0.0026	195.2 (±221.3)	0.39
	Mavs^−/−	335.5 (±435.0)	0.98	946.6 (±903.8)	0.0043
MIP-1α	WT	1.6 (±1.5)		2.1 (±1.9)
	Ifnar^−/−	8.8 (±6.6)	0.004
	Irf3^−/− × Irf7^−/−	5.8 (±3.6)	0.0001	5.6 (±5.1)	0.13
	Mavs^−/−	2.7 (±5.2)	0.52	6.6 (±6.8)	0.12
MIP-1β	WT	36.6 (±22.8)		45.2 (±20.4)
	Ifnar^−/−	35.0 (±24.0)	0.91
	Irf3^−/− × Irf7^−/−	23.7 (±12.3)	0.21	38.8 (±36.6)	0.71
	Mavs^−/−	31.5 (±16.2)	0.69	18.5 (±16.9)	0.03
RANTES	WT	14.5 (±5.8)		14.1 (±5.1)
	Ifnar^−/−	85.6 (±52.3)	0.0008
	Irf3^−/− × Irf7^−/−	50.9 (±34.5)	0.0001	12.6 (±7.6)	0.64
	Mavs^−/−	15.3 (±17.1)	0.30	31.6 (±19.8)	0.04
TNF-α	WT	276.4 (±193.8)		334.2 (±182.7)
	Ifnar^−/−	198.4 (±117.9)	0.36
	Irf3^−/− × Irf7^−/−	179.1 (±113.4)	0.20	145.2 (±101.3)	0.05
	Mavs^−/−	155.0 (±157.2)	0.17	137.3 (±114.5)	0.04

The indicated strains of mice were infected with OROV. Serum was collected on days 4 and 6 postinfection (p.i.), and cytokines and chemokines were measured by Bio-Plex array. Data represent the means (±SD) in pg/ml from 8 to 12 mice per group. Statistical significance was determined using the Mann-Whitney test, and P values were obtained after comparison to infected WT mice.