FIG 1.

Characterization of recombinant viruses. (A) RT-PCR products of the full-length S segment of wtUUKV and rUUKV and digestion with XhoI. Digestion was seen only with the amplified DNA derived from rUUKV, where a silent mutation creating an XhoI site was introduced at position 963. (B) Schematic diagrams of wild-type and mutant S segments lacking the NSs gene. (C) RT-PCR products of full-length S segments from rUUKV, rUUKdelNSsGFP, and rUUKdelNSs showing the size differences corresponding to the structures shown in panel B. (D) Western blot detection of UUKV N and NSs proteins in BSR cells infected with wtUUKV, rUUKV, rUUKdelNSsGFP, or rUUKdelNSs. Cells were infected at an MOI of 5 or mock infected, and cell lysates were prepared at 18 h p.i. Western blots were probed with antitubulin (dilution, 1:5,000), anti-UUKV NSs (dilution, 1:1,000), and anti-UUKV N (dilution, 1:100) antibodies.