FIG 3.

Growth properties of wtUUKV, rUUKV, rUUKdelNSs, and rUUKdelNSsGFP in BHK-21 cells. Cells were infected at an MOI of 0.1 FFU/ml, and cell culture medium was harvested or cell lysates were prepared at the time points indicated. (A) Growth curves. Infections were carried out in duplicate, and error bars represent the standard errors of the means for a representative experiment. (B) Viral protein synthesis. Cell lysates from the growth curve samples were harvested, and Western blots were probed with antitubulin, anti-UUKV NSs, and anti-UUKV N antibodies, as indicated.