FIG 1.

Most cell clones with resistance to HCV were CD81 defective and HCV nonpermissive. Huh7.5.1-derived cell clones (751r-1 and 751r-2) with resistance to HCV, which were isolated by the screen described in Materials and Methods, were transfected with the pcDNA3.1-hCD81 vector and cultured for 2 days. (A) Cell lysates were subjected to immunoblotting to detect the GAPDH and CD81 proteins. (B) Transfected cells and control Huh7.5.1-8 cells were infected with HCVcc and stained with anti-HCV core protein (green) and DAPI (blue) at 3 dpi.