TABLE 3.

Analysis of splicing events in early transcripts produced in organotypic raft culture^a

Primers	Splice(s)	Product size (bp)	Fold change				Inferred ORFs
			Donor 1	P	Donor 2	P
121/295	Unspliced Early	175	1.65 ± 0.27b	0.03	2.47 ± 0.57	0.03	E6, E7
E1F1/E4R	929^3434	190	1.049 ± 0.086	0.58	1.058 ± 0.26	0.83	E1^E4, E5
121/3517	233^416, 929^3434	708	1.03 ± 0.1	0.75	0.98 ± 0.008	0.07	E6*I, E7, E1^E4, E5
121/3517	233^3434	195	0.37 ± 0.087c	0.0008	0.59 ± 0.11	0.009	E6*II, E5

^aRNA extracted from 14-day-old raft cultures was converted to cDNA and amplified with the indicated primer pairs. The fold change in transcript level compared to that of wild-type HPV18 rafts is shown as the means and standard errors from three independent repeats. Significance (P) was calculated using Student's t test.

^bSignificant increase in expression levels.

^cSignificant decrease in expression levels compared to wild type.