FIG 6.

HCMV efficiently silences IE1 protein expression during a latent infection in cells with reduced levels of Rb. THP-1 cells in which Scr, Rb2, and Rb3 had been knocked down were mock treated or pretreated with valproic acid (VPA) 3 h prior to infection with AD169 IE2-GFP at an MOI of 1. Eighteen hours postinfection, mock-infected (M) cells and infected cells treated with VPA (+) or not treated with VPA (−) were harvested, and equal amounts of protein lysates were analyzed by Western blotting with the indicated antibodies. Data are representative of two biological replicates.