FIG 4.

Inhibition of CSFV infection in PK-15 cells by soluble LamR, laminin, or anti-LamR antibodies. (A, B) Influence of LamR on infection of CSFV and PRV in PK-15 cells (A) or SK6 cells (B). CSFV or PRV was incubated with different concentrations of LamR or BSA for 1 h at 4°C, followed by infection of the cells. The infected cell culture supernatant was collected at 48 hpi and titrated. (C, D) Influence of laminin on the invasion of CSFV and PRV in PK-15 cells (C) or SK6 cells (D). Cells were incubated with different concentrations of laminin or BSA for 1 h at 37°C, followed by infection with CSFV or PRV. The infected cell culture supernatant was collected at 48 hpi and titrated. (E, F) Antibody inhibition assay. PK-15 cells (E) or SK6 cells (F) were preincubated with anti-LamR antibodies or irrelevant rabbit IgG, followed by infection with CSFV or PRV. In the assays whose results are presented in panels C to F, the cells were infected with the indicated virus at an MOI of 1 and the supernatant was collected at 24 hpi. Bars represent standard deviations. *, P < 0.05; **, P < 0.01.