Cells treated with heparinase I showed distinct susceptibility to CSFV. (A) Relative infection rate of CSFV-NproFluc in PK-15 and SK6 cells treated with heparinase I; (B) relative infection rate of CSFV-NproFluc in SK6-LamR, SK6-EGFP, and SK6 cells treated with heparinase I; (C) relative infection rate of CSFV-NproFluc in PK-LamR, PK-EGFP, and PK-15 cells treated with heparinase I; (D) relative infection rate of CSFV-NproFluc in SK6-shLamR, SK6-shScr, and SK6 cells treated with heparinase I; (E) relative infection rate of CSFV-NproFluc in PK-shLamR, PK-shScr, and PK-15 cells treated with heparinase I. The relative infection rate was defined as the ratio of the rate of infection of cells treated with heparinase I to the rate of infection of cells not treated with heparinase I for the same cell line. Each sample was run in triplicate.