FIG 7.

Impaired innate and adaptive immunity in BAFFR-deficient mice during LCMV infection. WT and Baffr^−/− mice were infected with 200 PFU of LCMV-Docile. (A) Virus titers were measured in spleen, liver, lung, kidney, brain, and spinal cord tissues 72 h after infection (n = 5 or 6). (B) IFN-α concentrations were determined in the sera of infected animals 72 h following infection (n = 5 or 6). (C) Twenty days after infection, virus-specific CD8⁺ T cells were examined by tetramer (tet) staining in spleen tissues of mice (n = 5 or 6). (D) IFN-γ production of T cells as assessed by intracellular-cytokine staining and flow cytometric analysis was measured after in vitro restimulation with the MHC-I peptides gp33 and np396 (left) and the MHC-II peptide gp61 (right) (n = 5 or 6). (E) Virus titers were measured in spleen, liver, lung, kidney, brain, and spinal cord tissues 20 days after infection (n = 5 or 6). The error bars show SEM; n.s., not significant; the dashed line indicates the detection limit.