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. 2015 Apr 20;9:146. doi: 10.3389/fncel.2015.00146

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Copyright © 2015 Yamagishi, Yamada, Sawada, Nakano, Mori, Sawamoto and Sato.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of netrin-5 in the adult brain. (A) Immunoblotting analysis revealed that anti-netrin-5 antibody recognizes a protein of ~46 kDa in the total cell lysate of the dissected adult rat brains. Netrin-5 expression was higher in the olfactory bulb and lower in the hippocampus. The same membrane was reprobed with anti-GAPDH antibody as a loading control (lower panel). (B–E) Immunohistochemistry of sagittal sections of adult rat brain with anti-netrin-5 antibody. Anti-netrin-5 antibody stained strongly in the GCL of the OB (B), SVZ, RMS, CP (C), and SGZ of the DG (D,E). AO, anterior olfactory nucleus; CBL, cerebellum; CC, corpus callosum; CP, choroid plexus; CTX, cerebral cortex; DG, dentate gyrus; GCL, granule cell layer; HP, hippocampus; OB, olfactory bulb; RMS, rostral migratory stream; SGZ, subgranular zone; SVZ, subventricular zone. Bars indicate 500 μm in (B–D), and 100 μm in (E).