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. 2015 Apr 20;9:145. doi: 10.3389/fncel.2015.00145

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Copyright © 2015 Rietveld, Stuss, McPhee and Delaney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Single cells microinjected with Alexa Fluor 594 dye. Neurons selected for filling were distributed across brain slices to maximize the total number of visible cells per slice while preventing overlap of dendrites. Scale bar = 100 μm.