Table 1.

The primers used in PCR running

Species	Primer Name	Primer sequences	Amplicon size (bp)	Annealing temperature
		(5′→□3′)
E. acervulina	Ac-01F	AGTCAGCCACACAATAATGGCAAACATG	811	60°C
	Ac-01R	AGTCAGCCACAGCGAAAGACGTATGTG
E. brunetti	Br-01F	TGGTCGCAGAACCTACAGGGCTGT	626	63°C
	Br01R	TGGTCGCAGACGTATATTAGGGGTCTG
E. tenella	Tn-01F	CCGCCCAAACCAGGTGTCACG	539	60°C
	Tn-01R	CCGCCCAAACATGCAAGATGGC
E. praecox	Pr-01F	AGTCAGCCACCACCAAATAGAACCTTGG	354	58°C
	Pr-01R	GCCTGCTTACTACAAACTTGCAAGCCCT
E. mitis	Mt-01F	TATTTCCTGTCGTCGTCTCGC	327	57°C
	Mt-01R	GTATGCAAGAGAGAATCGGGA
E. maxima	Mx-01F	GGGTAACGCCAACTGCCGGGTATG	272	58°C
	Mx-01R	AGCAAACCGTAAAGGCCGAAGTCCTAGA
E. necatrix	Nc-01F	TTCATTTCGCTTAACAATATTTGGCCTCA	200	57°C
	Nc-01R	ACAACGCCTCATAACCCCAAGAAATTTTG

F = foward primer; R = reverse primer.