Table 2.
Factorial design for screening of significant independent variables affecting lipase production with the observed lipase activity values.
| X1 pH |
X2 temp (°C) |
X3 time (h) |
X4 FWPH (mg/mL) |
X5 FWO (%v/v) |
Y
a
(U/mL) |
Y
b
(U/mL) |
|---|---|---|---|---|---|---|
| 5 | 37 | 24 | 0.1 | 1 | 645 | 97.33 |
| 5 | 37 | 24 | 0.1 | 5 | 983 | 269.56 |
| 5 | 37 | 24 | 0.2 | 1 | 865.976 | 73.54 |
| 5 | 37 | 24 | 0.2 | 5 | 1428 | 391 |
| 5 | 37 | 72 | 0.1 | 1 | 243.98 | 41.2 |
| 5 | 37 | 72 | 0.1 | 5 | 1109.77 | 215.23 |
| 5 | 37 | 72 | 0.2 | 1 | 639 | 51.823 |
| 5 | 37 | 72 | 0.2 | 5 | 1208.67 | 287.45 |
| 5 | 47 | 24 | 0.1 | 1 | 908.743 | 158 |
| 5 | 47 | 24 | 0.1 | 5 | 1139.89 | 276 |
| 5 | 47 | 24 | 0.2 | 1 | 1097 | 89.779 |
| 5 | 47 | 24 | 0.2 | 5 | 1565.09 | 234 |
| 5 | 47 | 72 | 0.1 | 1 | 367.8188 | 68.9 |
| 5 | 47 | 72 | 0.1 | 5 | 890.098 | 213.856 |
| 5 | 47 | 72 | 0.2 | 1 | 737.51 | 49 |
| 5 | 47 | 72 | 0.2 | 5 | 1183 | 330 |
| 7 | 37 | 24 | 0.1 | 1 | 410 | 89.12 |
| 7 | 37 | 24 | 0.1 | 5 | 1430 | 274.33 |
| 7 | 37 | 24 | 0.2 | 1 | 1123 | 121 |
| 7 | 37 | 24 | 0.2 | 5 | 1603.45 | 409.66 |
| 7 | 37 | 72 | 0.1 | 1 | 378 | 34.567 |
| 7 | 37 | 72 | 0.1 | 5 | 967 | 208 |
| 7 | 37 | 72 | 0.2 | 1 | 809.09 | 58.526 |
| 7 | 37 | 72 | 0.2 | 5 | 1118 | 281 |
| 7 | 47 | 24 | 0.1 | 1 | 1010 | 91.23 |
| 7 | 47 | 24 | 0.1 | 5 | 1324 | 299 |
| 7 | 47 | 24 | 0.2 | 1 | 1238.45 | 145.89 |
| 7 | 47 | 24 | 0.2 | 5 | 1365.23 | 391.9 |
| 7 | 47 | 72 | 0.1 | 1 | 733 | 89.98 |
| 7 | 47 | 72 | 0.1 | 5 | 889 | 195 |
| 7 | 47 | 72 | 0.2 | 1 | 900.23 | 96 |
| 7 | 47 | 72 | 0.2 | 5 | 1203 | 310 |
X1: pH; X2: temperature, °C; X3: time, hours; X4: FWPH concentration (mg/mL); X5: FWO concentration, % v/v; Y a: lipase activity (U/mL) of MTCC 5695; Y b: lipase activity of MTCC 11361; FWPH: fish waste protein hydrolysates; FWO: fish waste oil.