Figure 2.

Sch B induces cell cycle arrest in mouse AML-12 and RAW 264.7 cells.

Notes: Cells were treated with Sch B at 0.1, 1, 10, and 25 μM for 24 hours and then subjected to flow cytometric analysis. The PI-stained DNA fluorescence intensity is shown in histogram overlay for cell cycle distribution of AML-12 and RAW 264.7 cells (A). The bar graphs show the percentage of mouse AML-12 and RAW 264.7 cells in G₁, S, and G₂/M phases (B). Data are the mean ± SEM of three independent experiments. *P<0.05; **P<0.01; and ***P<0.001 by one-way ANOVA.

Abbreviations: Sch B, schisandrin B; SEM, standard error of the mean; ANOVA, analysis of variance; PI, propidium iodide.