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. 2015 Apr 9;9:2001–2027. doi: 10.2147/DDDT.S77071

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© 2015 Zhang et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License

The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.

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Sch B modulates the expression levels of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 in mouse AML-12 and RAW 264.7 cells.

Notes: Cells were treated with Sch B at 0.1, 1, 10, and 25 μM for 24 hours and then the protein samples were subjected to Western blotting assay. Representative blots of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 (A). Bar graphs show the relative expression levels of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 in mouse AML-12 and RAW 264.7 cells (B). β-Actin was used as the internal control. Data are the mean ± SEM of three independent experiments. *P<0.05 and **P<0.01 by one-way ANOVA.

Abbreviations: Sch B, schisandrin B; SEM, standard error of the mean; ANOVA, analysis of variance; CDK2, cyclin-dependent kinase 2; Chk1, checkpoint kinase 1; PCNA, proliferating cell nuclear antigen; E2F1, E2F transcription factor 1.

Sch B modulates the expression levels of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 in mouse AML-12 and RAW 264.7 cells.

Notes: Cells were treated with Sch B at 0.1, 1, 10, and 25 μM for 24 hours and then the protein samples were subjected to Western blotting assay. Representative blots of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 (A). Bar graphs show the relative expression levels of CDK2, cyclin B1, cyclin D1, p27 Kip1, Chk1, PCNA, and E2F1 in mouse AML-12 and RAW 264.7 cells (B). β-Actin was used as the internal control. Data are the mean ± SEM of three independent experiments. *P<0.05 and **P<0.01 by one-way ANOVA.

Abbreviations: Sch B, schisandrin B; SEM, standard error of the mean; ANOVA, analysis of variance; CDK2, cyclin-dependent kinase 2; Chk1, checkpoint kinase 1; PCNA, proliferating cell nuclear antigen; E2F1, E2F transcription factor 1.