Table 2.

PCR amplification conditions for antisense and RNAi constructs.

Construct	Predenaturation	Denaturation	Annealing	Primer extension	Final extension	PCR cycle
pASACO2E1	2 min, 94°C	45 sec, 94°C	1 min, 62°C	30 sec, 72°C	10 min, 72°C	35
pRNAiACO1	3 min, 94°C	1 min, 94°C	45 sec, 60°C	1 min, 72°C	10 min, 72°C	35
pRNAiACO2
pRNAiCACO