Table 6.
Fiber separation and strength by purified enzymes.
| Enzyme formulation1 | Fried test score | Strength |
|---|---|---|
| Endopolygalacturonase (EPG) | 2.3 ± 0.4 | 29.7 ± 3.3 |
| EPG + xylanase (xyl) | 2.4 ± 0.5 | 29.8 ± 5.3 |
| EPG + endoglucanase (EG) | 2.3 ± 0.5 | 26.9 ± 3.7 |
| EPG + Xyl + EG | 2.0 ± 0 | 22.3 ± 1.6 |
| Xyl | 1.1 ± 0.2 | ND |
| EG | 1.4 ± 0.5 | ND |
| EPG + Xyl +EG + Celluclast | 2.4 ± 0.2 | ND |
1EPG is endopolygalacturonase purified from Rhizopus oryzae sb (NRRL 29086) used at 18.1 U/mL to equal pectinase activity in SP 240; Xyl is a cloned xylanase from Thermatoga maritima [29]; EG is endoglucanase Novozymes SP 613; Celluclast is a Novozymes cellulase from Trichoderma reesei ATCC26921. All enzyme mixtures contained 50 mmol oxalic acid as chelator.
Data from [30].