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Reversibility of cosolvent's induced enhancement of α-amylase activity. The enzyme activity was measured after treatment with cosolvents and removal of cosolvents from the protein solution.
| Enzyme activity (×1000 unit) | |||
|---|---|---|---|
| Cosolvents | Without any treatment | After treatment with cosolvents (40%, w/v) | After removal of cosolvents |
| Buffer only | 2.6 ± 0.15 | ||
| Glycerol | 3.6 ± 0.1 | 2.6 ± 0.25 | |
| Sorbitol | 3.7 ± 0.3 | 2.8 ± 0.15 | |
| Sucrose | 3.8 ± 0.25 | 2.4 ± 0.1 | |
| Trehalose | 4.1 ± 0.3 | 2.7 ± 0.2 | |
