Skip to main content
. 2015 Apr 6;197(9):1614–1623. doi: 10.1128/JB.02523-14

FIG 2.

FIG 2

The c0507-c0506-c0505 gene cluster and agarose gel electrophoresis of the RT-PCR products. (A) The c0507-c0506-c0505 gene cluster. The double-headed arrows show the deleted parts in the corresponding mutants: a 652-bp fragment, ranging from base 378 to base 1029 of c0507, was removed in the Δc0507 strain; a 292-bp fragment, ranging from base 271 to base 562 of c0506, was removed in the Δc0506 strain; and a 221-bp fragment, ranging from base 318 to base 538 of c0505, was removed in the Δc0505 strain. (B) Agarose gel electrophoresis of the RT-PCR product. (Panel a) The genomic DNA and total RNA were isolated from wild-type Ha. japonica. (Panel b) The genomic DNA and total RNA were isolated from the Δc0506 strain. (Panel c) The genomic DNA and total RNA were isolated from the Δc0507 strain. (Panel d) The genomic DNA and total RNA were isolated from the Δc0505 strain. Lanes 1, 4, and 7, the positive-control reaction product using genomic DNA as the template; lanes 2, 5, and 8, the RT-PCR product; lanes 3, 6, and 9, the negative-control reaction product with total RNA not subjected to reverse transcription.