FIG 3.

Cell suspension color of the wild type and of the mutants and their genetically modified Ha. japonica strains, and HPLC elution profiles of carotenoids extracted from them. (A) Wild type. (B) Δc0506 strain. (C) Δc0506(pJc0506) strain. (D) Δc0507 strain. (E) Δc0507(pJc0507) strain. (F) Δc0505 strain. (G) Δc0505(pJc0505) strain. Peak 1, bacterioruberin (BR); peak 2, monoanhydrobacterioruberin (MABR); peak 3, bisanhydrobacterioruberin (BABR); peak 4, isopentenyldehydrorhodopin (IDR); peak 5, lycopene; peak 4′, tetrahydrobisanhydrobacterioruberin (TH-BABR); peak 5′, dihydroisopentenyldehydrorhodopin (DH-IDR). Peaks 1′, 2′, 3′, 6′, and 7′ might be derivatives of lycopene precursors. The eluent was methanol-water (9:1 [vol/vol]) for the first 10 min and then 100% methanol (1.5 ml/min).