TABLE 2.
Sequences of the primers used in this study
| Primer | Sequencea | Usage |
|---|---|---|
| c0507-S | 5′-GGTTGGCCTCCAGCTCATTG-3′ | To confirm the transcription of target genes by RT-PCR |
| c0507-A | 5′-CTCGTGGTCCGGCAGGAGTTC-3′ | |
| c0506-S | 5′-TCGCCCTGTTTCTGTACTTCAC-3′ | |
| c0506-A | 5′-GATATCTGGAATCGCCGAGAAGG-3′ | |
| c0505-S | 5′-CACCGAGAACCGGTTTACCATC-3′ | |
| c0505-A | 5′-ACTGCGGCATCTCGTAGATCC-3′ | |
| c0507-S1 | 5′-ATGTGGCCACGAATGCCATAC-3′ | To amplify the target genes by PCR for the construction of mutants |
| c0507-A1 | 5′-GGTCTGTCGGGAGTTCTGGC-3′ | |
| c0506-S1 | 5′-GGACATCGCCTGAGTAATGCC-3′ | |
| c0506-A1 | 5′-GGTCTTTGCCGCTACCCATAC-3′ | |
| c0506-S3 | 5′-TCTGGGCGATGGGGATGCAC-3′ | |
| c0506-A3 | 5′-TTTCGGATTGTGCTCGTCGATATCGG-3′ | |
| c0505-S1 | 5′-GCTGTATGGGTAGCGGCAAAG-3′ | |
| c0505-A1 | 5′-GAGCTGGCTGATTCCAAACGG-3′ | |
| c0505-S3 | 5′-TGGATCCTGGAGCCGTAGCTATC-3′ | |
| c0505-A3 | 5′-AGCTCGATGCCGTAGGAGTACAG-3′ | |
| c0507-S4 | 5′-GAATTCCATATGAGTGACTTGTCCGGTG-3′ | To amplify the intact target genes by PCR for the complementation study |
| c0507-A4 | 5′-GGGGATCCTCATTAGTGGTGGTGGTGGTGGTGGGCGATGTCCTCGATGAGC-3′ | |
| c0506-S4 | 5′-GAATTCCATATGCCAGAACTCCCGACAG-3′ | |
| c0506-A4 | 5′-GGGGATCCTCATTAGTGGTGGTGGTGGTGGTGCCCATACAGCATCACCCAC-3′ | |
| c0505-S4 | 5′-GAATTCCATATGGGTAGCGGCAAAGACC-3′ | |
| c0505-A4 | 5′-GGCCATGGTCATTAGTGGTGGTGGTGGTGGTGCCGCCAGACGGCCCGACCG-3′ |
a
The introduced NdeI, BamHI, and NcoI restriction sites are underlined.