FIG 2.

Western blot analysis of complementation experiments. Whole-cell lysates from mmp0350 (A), mmp0351 (B), mmp0352 (C), mmp0353 (D), and mmp0357 (E) deletion mutants as well as the same deletion mutants harboring their respective complementation vectors were separated by SDS-PAGE, transferred to an Immobilon membrane, and probed with anti-FlaB2. Complemented strains were grown in N-free medium with the addition of either alanine (transcription on) or NH₄Cl (transcription off). Wild-type cell lysates (Mm900) were run as the control.