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Spot test plates of P. aeruginosa strains using bacteriophage D3. Quadrants marked “D3” were spotted with 10 μl bacteriophage D3 (2 × 1010 PFU/ml stock). Quadrants marked “LB” were spotted with 10 μl of LB medium as a negative control. The complementation of the P. aeruginosa ΔwbpI mutant using MMP0357 expressed in trans restored D3 sensitivity.
