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Restoration of O5 OSA synthesis in the complemented P. aeruginosa ΔwbpI strain. (A) Silver staining of LPS isolated from P. aeruginosa strains; (B) Western blot of LPS isolated from P. aeruginosa strains; the blot was developed using MF15-4, antibodies specific for O5 OSA. The complementation of the P. aeruginosa ΔwbpI mutant using MMP0357 expressed in trans restored O5 OSA biosynthesis.
