TABLE 2.
Strain | ywaEb | β-Galactosidase activitya |
Fold changec | |
---|---|---|---|---|
ywaE-lacZ | ywaEopA7G-lacZ | |||
BR151 | + | 0.73 ± 0.15 | 92 ± 16 | 130 |
ZKO | + | 0.83 ± 0.23 | 71 ± 3.2 | 86 |
SKO-EopA7G | + | 1.0 ± 0.20 | 90 ± 19 | 90 |
SKO-EΩ4 | − | 37 ± 6.2 | 310 ± 43 | 8.4 |
EΩ4 | − | 82 ± 5.2 | 260 ± 9.3 | 3.2 |
Fusions were integrated in single copy into B. subtilis strains. Cells were grown in minimal medium until late exponential phase and then assayed for β-galactosidase activity (Miller units). The values reported are averages of three repeats ± standard errors.
Indicates if the strain has a functional ywaE gene.
Fold change is the ratio of β-galactosidase activity for ywaEopA7G-lacZ to ywaE-lacZ.