TABLE 2.

Expression of ywaE-lacZ and ywaE^opA7G-lacZ fusions

Strain	ywaEb	β-Galactosidase activitya		Fold changec
		ywaE-lacZ	ywaEopA7G-lacZ
BR151	+	0.73 ± 0.15	92 ± 16	130
ZKO	+	0.83 ± 0.23	71 ± 3.2	86
SKO-EopA7G	+	1.0 ± 0.20	90 ± 19	90
SKO-EΩ4	−	37 ± 6.2	310 ± 43	8.4
EΩ4	−	82 ± 5.2	260 ± 9.3	3.2

^aFusions were integrated in single copy into B. subtilis strains. Cells were grown in minimal medium until late exponential phase and then assayed for β-galactosidase activity (Miller units). The values reported are averages of three repeats ± standard errors.

^bIndicates if the strain has a functional ywaE gene.

^cFold change is the ratio of β-galactosidase activity for ywaE^opA7G-lacZ to ywaE-lacZ.