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. Author manuscript; available in PMC: 2015 Apr 20.
Published in final edited form as: Mol Cell. 2014 Mar 20;53(6):1020–1030. doi: 10.1016/j.molcel.2014.02.027

Figure 4. Oligouridylation occurs on transfected, capped, non-polyadenylated RNAs.

Figure 4

(A–B) BTH-capped luciferase mRNA ending in a histone stemloop (LUC-SL, panel A) or tetraloop (LUC-TL, panel B) was nucleoporated into synchronized HeLa cells that were then treated with HU, and RNA was isolated 30 min after HU treatment. These are the same RNA samples that were previously analyzed (Su et al., 2013). Results with a set of ARCA capped mRNAs is shown in Supp. Fig. 4.

(C) Possible pathways of histone mRNA degradation initiated at the 3′ end of histone mRNA.